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KCNA5/KV1-5 Antibody (YA6623)

(Synonyms: ATFB7 antibody; cardiac potassium channel antibody; HCK1 antibody; HK2 antibody; HPCN1 antibody; insulinoma and islet potassium channel antibody; KCNA5 antibody; KCNA5_HUMAN antibody; KV1.5 antibody; PCN1 antibody; ATFB7 antibody; cardiac potassium channel antibody; HCK1 antibody; HK2 antibody; HPCN1 antibody; insulinoma and islet potassium channel antibody; KCNA5 antibody; KCNA5_HUMAN antibody; KV1.5 antibody; PCN1 antibody; potassium channel 1 antibody; Potassium channel, insulinoma and islet cell antibody; Potassium channel, voltage-gated, shaker-related subfamily, member 5 antibody; Potassium voltage gated channel subfamily A member 5 antibody; Potassium voltage-gated channel subfamily A member 5 antibody; Voltage-gated potassium channel HK2 antibody; voltage-gated potassium channel protein Kv1.5 antibody; Voltage-gated potassium channel subunit Kv1.5 antibody; )
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Based on 1 Customer Validation

KCNA5/KV1-5 Antibody (YA6623) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KCNA5/KV1-5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for KCNA5/KV1-5 Antibody (YA6623)
  • KCNA5/KV1-5 Antibody (YA6623) Product Image
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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:2000

Product Details

Description

KCNA5/KV1-5 Antibody (YA6623) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to KCNA5/KV1-5.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 67 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 67 kDa
Immunogen

Synthetic peptide within rat KCNA5 aa 249-298 / 602.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for KCNA5/KV1-5 Antibody (YA6623)
    Western blot analysis was performed on protein extracts (30 μg) from Rat brain (lane 2),Mouse brain (lane 3), SH-SY5Y (lane 4), and Mouse kidney (lane 5) using KCNA5/KV1-5 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    KCNA5/KV1-5 is a Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel. Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation. Homotetrameric channels display rapid activation and slow inactivation. Required for normal electrical conduction including formation of the infranodal ventricular conduction system and normal action potential configuration, as a result of its interaction with XIRP2. May play a role in regulating the secretion of insulin in normal pancreatic islets|Exhibits a faster depolarization rate, reduced voltage-dependent recovery from inactivation and an excessive cumulative inactivation[1][2].

  • Subcellular Localization

    Cell membrane

  • Expression


    Tissue_Specificity: Pancreatic islets and insulinoma

  • Isoforms & Post-Translational Modification

    P22460 has two isomers: P22460-1: 67228 Da (predicted); P22460-2: 44427 Da (predicted).
    Glycosylated丨Sumoylated on Lys-221, and Lys-536, preferentially with SUMO3

  • Subunit

    Homotetramer and heterotetramer of potassium channel proteins (PubMed:20018952)

  • SwissProt ID

    P22460

  • Gene ID
    3741 [NCBI]
  • Synonyms

    ATFB7 antibody; cardiac potassium channel antibody; HCK1 antibody; HK2 antibody; HPCN1 antibody; insulinoma and islet potassium channel antibody; KCNA5 antibody; KCNA5_HUMAN antibody; KV1.5 antibody; PCN1 antibody; ATFB7 antibody; cardiac potassium channel antibody; HCK1 antibody; HK2 antibody; HPCN1 antibody; insulinoma and islet potassium channel antibody; KCNA5 antibody; KCNA5_HUMAN antibody; KV1.5 antibody; PCN1 antibody; potassium channel 1 antibody; Potassium channel, insulinoma and islet cell antibody; Potassium channel, voltage-gated, shaker-related subfamily, member 5 antibody; Potassium voltage gated channel subfamily A member 5 antibody; Potassium voltage-gated channel subfamily A member 5 antibody; Voltage-gated potassium channel HK2 antibody; voltage-gated potassium channel protein Kv1.5 antibody; Voltage-gated potassium channel subunit Kv1.5 antibody;

KCNA5/KV1-5 Antibody (YA6623) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from Rat brain (lane 2),Mouse brain (lane 3), SH-SY5Y (lane 4), and Mouse kidney (lane 5) using KCNA5/KV1-5 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • KCNA5/KV1-5 Antibody (YA6623) Product Image
    1/16
  • Experimental Validation Results for KCNA5/KV1-5 Antibody (YA6623)
  • KCNA5/KV1-5 Antibody (YA6623) Product Image
  • Experimental Validation Results for KCNA5/KV1-5 Antibody (YA6623)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (30 μg) from Rat brain (lane 2),Mouse brain (lane 3), SH-SY5Y (lane 4), and Mouse kidney (lane 5) using KCNA5/KV1-5 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for KCNA5/KV1-5 Antibody (YA6623)
  • KCNA5/KV1-5 Antibody (YA6623) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (30 μg) from Rat brain (lane 2),Mouse brain (lane 3), SH-SY5Y (lane 4), and Mouse kidney (lane 5) using KCNA5/KV1-5 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:2000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • KCNA5/KV1-5 Antibody (YA6623) Product Image WB