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Rac1-2-3 Antibody (YA6751)

(Synonyms: Cell migration inducing gene 5 protein antibody; EN 7 antibody; OTTMUSP00000004488 antibody; p21 Rac3 antibody; p21-Rac3 antibody; Rac1B antibody; RAC3 antibody; RAC3_HUMAN antibody; RAS related C3 botulinum substrate 3 antibody; Ras related C3 botulinum toxin substrate 3 (rho family small GTP binding protein Rac3) antibody; Cell migration inducing gene 5 protein antibody; EN 7 antibody; OTTMUSP00000004488 antibody; p21 Rac3 antibody; p21-Rac3 antibody; Rac1B antibody)
Customer Review

Based on 1 Customer Validation

Rac1-2-3 Antibody (YA6751) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Rac1-2-3.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IF-Tissue, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Rac1-2-3 Antibody (YA6751)
  • Experimental Validation Results for Rac1-2-3 Antibody (YA6751)
  • Experimental Validation Results for Rac1-2-3 Antibody (YA6751)
  • Rac1-2-3 Antibody (YA6751) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:2000 1:50-1:200 1:200 1:200

Product Details

Description

Rac1-2-3 Antibody (YA6751) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Rac1-2-3.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 21 kDa
Immunogen

Recombinant full length protein of Human RAC1 aa 1-192 / 192.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719253

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Rac1-2-3 Antibody (YA6751)
    Western blot analysis of extracts from MCF-7 (lane 2(20μg) and MCF-7 (lane 3(40μg) using Rac1-2-3 (HY-P87058) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Rac1-2-3 Antibody (YA6751)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Rac1-2-3 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Rac1-2-3 Antibody (YA6751)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Rac1-2-3 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Rac1-2-3 is a Plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In its active state, binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. Regulatory subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-))[1][2].

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_Specificity: Hematopoietic specific

  • Isoforms & Post-Translational Modification

    P15153: 192 amino acids, molecular weight 21429 Da.

  • Subunit

    Interacts with DOCK2, which may activate it

  • SwissProt ID

    P15153

  • Gene ID
    5880 [NCBI]
  • Synonyms

    Cell migration inducing gene 5 protein antibody; EN 7 antibody; OTTMUSP00000004488 antibody; p21 Rac3 antibody; p21-Rac3 antibody; Rac1B antibody; RAC3 antibody; RAC3_HUMAN antibody; RAS related C3 botulinum substrate 3 antibody; Ras related C3 botulinum toxin substrate 3 (rho family small GTP binding protein Rac3) antibody; Cell migration inducing gene 5 protein antibody; EN 7 antibody; OTTMUSP00000004488 antibody; p21 Rac3 antibody; p21-Rac3 antibody; Rac1B antibody

Rac1-2-3 Antibody (YA6751) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane 2(20μg) and MCF-7 (lane 3(40μg) using Rac1-2-3 (HY-P87058) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Rac1-2-3 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Rac1-2-3 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Rac1-2-3 Antibody (YA6751) Product Image
    1/16
  • Experimental Validation Results for Rac1-2-3 Antibody (YA6751)
  • Experimental Validation Results for Rac1-2-3 Antibody (YA6751)
  • Experimental Validation Results for Rac1-2-3 Antibody (YA6751)
  • Rac1-2-3 Antibody (YA6751) Product Image
  • Experimental Validation Results for Rac1-2-3 Antibody (YA6751)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane 2(20μg) and MCF-7 (lane 3(40μg) using Rac1-2-3 (HY-P87058) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Rac1-2-3 Antibody (YA6751)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Rac1-2-3 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Rac1-2-3 Antibody (YA6751)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Rac1-2-3 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Rac1-2-3 Antibody (YA6751)
  • Experimental Validation Results for Rac1-2-3 Antibody (YA6751)
  • Experimental Validation Results for Rac1-2-3 Antibody (YA6751)
  • Rac1-2-3 Antibody (YA6751) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from MCF-7 (lane 2(20μg) and MCF-7 (lane 3(40μg) using Rac1-2-3 (HY-P87058) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Rac1-2-3 Antibody (YA6751) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Rac1-2-3 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Rac1-2-3 Antibody (YA6751) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using Rac1-2-3 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Rac1-2-3 Antibody (YA6751) Product Image WB
  • Rac1-2-3 Antibody (YA6751) Product Image IHC-P
  • Rac1-2-3 Antibody (YA6751) Product Image IHC-P