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mtTFA Antibody (YA3360)

(Synonyms: MtTF1; mtTFA; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM; Transcription factor 6)
Customer Review

Based on 1 Customer Validation

mtTFA Antibody (YA3360) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to mtTFA.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for mtTFA Antibody (YA3360)
  • Experimental Validation Results for mtTFA Antibody (YA3360)
  • Experimental Validation Results for mtTFA Antibody (YA3360)
  • mtTFA Antibody (YA3360) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200 1:20

Product Details

Description

mtTFA Antibody (YA3360) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to mtTFA.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 24 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 29 kDa
Species Reactivity Database

Entrez Gene: 7019 Human

SwissProt: Q00059 Human

Immunogen

A synthetic peptide of human mtTFA

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105722

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for mtTFA Antibody (YA3360)
    Western blot analysis of extracts from K562 (lane2, 20μg), HL-60 (lane3, 20μg), Hela (lane4, 20μg) and MCF-7 (lane5, 20μg) using mtTFA Antibody (HY-P83615). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for mtTFA Antibody (YA3360)
    Immunocytochemistry analysis of Hela cells labeling mtTFA Antibody (HY-P83615) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with mtTFA Antibody (HY-P83615) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for mtTFA Antibody (YA3360)
    Immunocytochemistry analysis of MCF-7 cells labeling mtTFA Antibody (HY-P83615) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with mtTFA Antibody (HY-P83615) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    mtTFA binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA[1][2][3][4][5][6][7][8][9].

  • Subcellular Localization

    Mitochondrion; Mitochondrion matrix, mitochondrion nucleoid

  • Isoforms & Post-Translational Modification

    Q00059 has 2 isomers: Q00059-1: 29097 Da (predicted); Q00059-2: 25466 Da (predicted).
    Phosphorylation by PKA within the HMG box 1 impairs DNA binding and promotes degradation by the AAA+ Lon protease

  • Subunit

    Monomer; binds DNA as a monomer (PubMed:19304746, PubMed:22037171, PubMed:22037172). Homodimer (PubMed:29149603). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT (PubMed:29149603). In this complex TFAM recruits POLRMT to the promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed:29149603). Upon metabolic stress, forms a complex composed of FOXO3, SIRT3, TFAM and POLRMT (PubMed:12897151, PubMed:29445193). Interacts with TFB1M and TFB2M (PubMed:12897151). Interacts with CLPX; this enhances DNA-binding (PubMed:22841477)

  • SwissProt ID

    Q00059

  • Gene ID
    7019 [NCBI]
  • Synonyms

    MtTF1; mtTFA; TCF6; TCF6L1; TCF6L2; TCF6L3; TFAM; Transcription factor 6

  • Research Field

    Tags & Cell Markers

[1]. Celestini V, et al. Uncoupling FoxO3A mitochondrial and nuclear functions in cancer cells undergoing metabolic stress and chemotherapy. Cell Death Dis. 2018 Feb 14;9(2):231. [Content Brief]

[2]. Hao Z, et al. N(6)-Deoxyadenosine Methylation in Mammalian Mitochondrial DNA. Mol Cell. 2020 May 7;78(3):382-395.e8. [Content Brief]

[3]. Hillen HS, et al. Structural Basis of Mitochondrial Transcription Initiation. Cell. 2017 Nov 16;171(5):1072-1081.e10. [Content Brief]

[4]. Litonin D, et al. Human mitochondrial transcription revisited: only TFAM and TFB2M are required for transcription of the mitochondrial genes in vitro. J Biol Chem. 2010 Jun 11;285(24):18129-33. [Content Brief]

[5]. Rubio-Cosials A, et al. Human mitochondrial transcription factor A induces a U-turn structure in the light strand promoter. Nat Struct Mol Biol. 2011 Oct 30;18(11):1281-9. [Content Brief]

[6]. Fisher RP, et al. DNA wrapping and bending by a mitochondrial high mobility group-like transcriptional activator protein. J Biol Chem. 1992 Feb 15;267(5):3358-67. [Content Brief]

[7]. Gangelhoff TA, et al. Structural analysis and DNA binding of the HMG domains of the human mitochondrial transcription factor A. Nucleic Acids Res. 2009 Jun;37(10):3153-64. [Content Brief]

[8]. Kasashima K, et al. Maintenance of mitochondrial genome distribution by mitochondrial AAA+ protein ClpX. Exp Cell Res. 2012 Nov 1;318(18):2335-43. [Content Brief]

[9]. Ngo HB, et al. The mitochondrial transcription and packaging factor Tfam imposes a U-turn on mitochondrial DNA. Nat Struct Mol Biol. 2011 Oct 30;18(11):1290-6. [Content Brief]

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mtTFA Antibody (YA3360) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane2, 20μg), HL-60 (lane3, 20μg), Hela (lane4, 20μg) and MCF-7 (lane5, 20μg) using mtTFA Antibody (HY-P83615). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling mtTFA Antibody (HY-P83615) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with mtTFA Antibody (HY-P83615) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling mtTFA Antibody (HY-P83615) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with mtTFA Antibody (HY-P83615) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • mtTFA Antibody (YA3360) Product Image
    1/16
  • Experimental Validation Results for mtTFA Antibody (YA3360)
  • Experimental Validation Results for mtTFA Antibody (YA3360)
  • Experimental Validation Results for mtTFA Antibody (YA3360)
  • mtTFA Antibody (YA3360) Product Image
  • Experimental Validation Results for mtTFA Antibody (YA3360)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane2, 20μg), HL-60 (lane3, 20μg), Hela (lane4, 20μg) and MCF-7 (lane5, 20μg) using mtTFA Antibody (HY-P83615). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for mtTFA Antibody (YA3360)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling mtTFA Antibody (HY-P83615) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with mtTFA Antibody (HY-P83615) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for mtTFA Antibody (YA3360)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling mtTFA Antibody (HY-P83615) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with mtTFA Antibody (HY-P83615) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for mtTFA Antibody (YA3360)
  • Experimental Validation Results for mtTFA Antibody (YA3360)
  • Experimental Validation Results for mtTFA Antibody (YA3360)
  • mtTFA Antibody (YA3360) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane2, 20μg), HL-60 (lane3, 20μg), Hela (lane4, 20μg) and MCF-7 (lane5, 20μg) using mtTFA Antibody (HY-P83615). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • mtTFA Antibody (YA3360) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling mtTFA Antibody (HY-P83615) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with mtTFA Antibody (HY-P83615) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • mtTFA Antibody (YA3360) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling mtTFA Antibody (HY-P83615) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with mtTFA Antibody (HY-P83615) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • mtTFA Antibody (YA3360) Product Image WB
  • mtTFA Antibody (YA3360) Product Image ICC
  • mtTFA Antibody (YA3360) Product Image ICC