Glutathione Reductase Antibody (YA403)

製品番号: HY-P80693: データシート SDS COA User Guide for Antibodies
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Glutathione Reductase Antibody (YA403) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glutathione Reductase.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$84	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

Glutathione Reductase Antibody (YA403) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Glutathione Reductase.

Host

Rabbit

Clonality

Recombinant, Monoclonal

分子量

Predicted band size: 56 kDa;

Observed band size: 51 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P00390

Gene ID

2936 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Glutathione Reductase.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	純度	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from HepG2(lane 2(20ug) , A549(lane 3(20ug) and K562(lane 4(20ug) using Glutathione Reductase Antibody (HY-P80693) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Catalyzes the reduction of glutathione disulfide (GSSG) to reduced glutathione (GSH). Constitutes the major mechanism to maintain a high GSH:GSSG ratio in the cytosol

Subcellular Localization：Mitochondrion; Cytoplasm

Isoforms & Post-Translational Modification：P00390 has 5 isomers: P00390-1: 56257 Da (predicted); P00390-2: 51701 Da (predicted); P00390-3: 53027 Da (predicted); P00390-4: 50497 Da (predicted); P00390-5: 47267 Da (predicted).

Subunit：Homodimer; disulfide-linked

RRID

AB_3102956

Database

Entrez Gene: 2936 Human ; 14782 Mouse ;

SwissProt: P00390 Human ; P47791 Mouse ; P70619 Rat

OMIM: 618660 Human

Research Field

Signal Transduction

Synonyms

GR; HEL-75; HEL-S-122m

ドキュメンテーション

Select Batch:

データシート (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)