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  3. KDM1A Antibody (YA718)

KDM1A Antibody (YA718)

Cat. No.: HY-P80731
COA User Guide for Antibodies

KDM1A Antibody (YA718) is a non-conjugated and Mouse origined monoclonal antibody about 93 kDa, targeting to KDM1A (1B2). It can be used for WB assays with tag free, in the background of Human, Mouse, Monkey.

For research use only. We do not sell to patients.

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10 μL $70 In-stock
50 μL $185 In-stock
100 μL $300 In-stock
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  • Biological Activity

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Description

KDM1A Antibody (YA718) is a non-conjugated and Mouse origined monoclonal antibody about 93 kDa, targeting to KDM1A (1B2). It can be used for WB assays with tag free, in the background of Human, Mouse, Monkey.

Background

KDM1A (1B2): This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Tag

Free

Gene ID

23028

Synonyms
KDM1A; AOF2; KDM1; KIAA0601; LSD1; Lysine-specific histone demethylase 1A; BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2
Molecular Weight

Predicted band size: 93 kDa;Observed band size: 95-110 kDa

Purity

affinity purified

Conjugation

Non-conjugated

Research Field

Epigenetics and Nuclear Signaling

Product Categories

Primary Antibody; Mouse Monoclonal Antibody

Host

Mouse

Reactivity

Human, Mouse, Monkey

SwissProt ID

O60341

Dilution Ratio

WB: 1:500-1:1,000

  • Western blot analysis of extracts from Jurkat(lane 2(40μg), HCT116(lane 3(40μg)) and NIH3T3(lane 4(40μg)), using KDM1A (HY-P80731) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80731, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.
Application

WB

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG1

Immunogen

Synthetic peptide corresponding to Human LSD1.The exact sequence is proprietary to MCE.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
KDM1A Antibody (YA718)
Cat. No.:
HY-P80731
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