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EBF1 Antibody (YA1938)

(Synonyms: COE1; EBF; Ebf1; O/E 1; OE1; OLF1)
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Based on 1 Customer Validation

EBF1 Antibody (YA1938) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EBF1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for EBF1 Antibody (YA1938)
  • EBF1 Antibody (YA1938) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

EBF1 Antibody (YA1938) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EBF1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 64 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 64 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human EBF1 aa50-90/591.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EBF1 Antibody (YA1938)
    Western blot analysis was performed on nuclear protein extracts (35 μg) from THP-1 (lane 2) using EBF1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody Lamin B1 Antibody (HY-P84174) (1:2000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    EBF1 is key pioneer transcription factor of B-cell specification and commitment. Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Operates in a transcription factor network to activate B-cell-specific genes and repress genes associated with alternative cell fates. For instance, positively regulates many B-cell specific genes including BCR or CD40 while repressing genes that direct cells into alternative lineages, including GATA3 and TCF7 for the T-cell lineage. In addition to its role during lymphopoiesis, controls the thermogenic gene program in adipocytes during development and in response to environmental cold; (Microbial infection) Acts as a chromatin anchor for Epstein-Barr virus EBNA2 to mediate the assembly of EBNA2 chromatin complexes in B-cells. In addition, binds to the viral LMP1 proximal promoter and promotes its expression during latency[1][2][3].

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    Q9UH73 has 2 isomers: Q9UH73-1: 64464 Da (predicted); Q9UH73-2: 61059 Da (predicted).

  • Subunit

    Homodimer (PubMed:20592035). Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes (PubMed:14630787). Interacts with CCR4-NOT component CNOT3 (PubMed:14630787)

  • SwissProt ID

    Q9UH73

  • Gene ID
    1879 [NCBI]
  • Synonyms

    COE1; EBF; Ebf1; O/E 1; OE1; OLF1

  • Research Field

    Epigenetics and Nuclear Signaling

[1]. Yang CY, et al. Interaction of CCR4-NOT with EBF1 regulates gene-specific transcription and mRNA stability in B lymphopoiesis. Genes Dev. 2016 Oct 15;30(20):2310-2324. [Content Brief]

[2]. Glaser LV, et al. EBF1 binds to EBNA2 and promotes the assembly of EBNA2 chromatin complexes in B cells. PLoS Pathog. 2017 Oct;13(10):e1006664. [Content Brief]

[3]. Murata T, et al. Induction of Epstein-Barr Virus Oncoprotein LMP1 by Transcription Factors AP-2 and Early B Cell Factor. J Virol. 2016 Apr;90(8):3873-3889. [Content Brief]

EBF1 Antibody (YA1938) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on nuclear protein extracts (35 μg) from THP-1 (lane 2) using EBF1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody Lamin B1 Antibody (HY-P84174) (1:2000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • EBF1 Antibody (YA1938) Product Image
    1/16
  • Experimental Validation Results for EBF1 Antibody (YA1938)
  • EBF1 Antibody (YA1938) Product Image
  • Experimental Validation Results for EBF1 Antibody (YA1938)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on nuclear protein extracts (35 μg) from THP-1 (lane 2) using EBF1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody Lamin B1 Antibody (HY-P84174) (1:2000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for EBF1 Antibody (YA1938)
  • EBF1 Antibody (YA1938) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on nuclear protein extracts (35 μg) from THP-1 (lane 2) using EBF1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody Lamin B1 Antibody (HY-P84174) (1:2000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • EBF1 Antibody (YA1938) Product Image WB