Muscarinic Acetylcholine Receptor M2 Antibody (YA2610)

Cat. No.: HY-P82865: Ficha de datos COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Muscarinic Acetylcholine Receptor M2 Antibody (YA2610) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Muscarinic Acetylcholine Receptor M2.

Para uso exclusivo en investigación. No vendemos a pacientes.

Tamaño	Stock	Cantidad
Muestra gratis	Apply now
10 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

or Consulta para venta a granel

* Seleccione Cantidad antes de agregar artículos.

Muscarinic Acetylcholine Receptor M2 Antibody (YA2610) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Descripciòn

Descripciòn

Muscarinic Acetylcholine Receptor M2 Antibody (YA2610) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Muscarinic Acetylcholine Receptor M2.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Peso molecular

Predicted band size: 52 kDa;

Observed band size: 52 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P08172

Gene ID

1129 [NCBI]

Immunogen

A synthesized peptide derived from human ACM2 aa152-180/466.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:50

Sensitivity	Endogenous	Pureza	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

ALL WB IHC-P

Western blot analysis of extracts from HepG2(lane 2(20ug) ，SH-SY5Y(lane 3(20ug) and U-87MG(lane 4(20ug) using Muscarinic Acetylcholine Receptor M2 Antibody (HY-P82865) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Muscarinic Acetylcholine Receptor M2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Muscarinic Acetylcholine Receptor M2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol

Subcellular Localization：Cell membrane; Multi-pass membrane protein; Postsynaptic cell membrane; Multi-pass membrane protein

Expression：
Induction:Up-regulated in response to enterovirus 71 (EV71) infection

Subunit：Interacts with ARRB1 and ARRB2. Interacts with RACK1; the interaction regulates CHRM2 internalization

RRID

AB_3104958

Database

Entrez Gene: 1129 Human ; 243764 Mouse ; 81645 Rat

SwissProt: P08172 Human ; Q9ERZ4 Mouse ; P10980 Rat

OMIM: 608516 Human

Research Field

Neuroscience

Synonyms

CHRM2; HM2; AChR; Acm2

Documentación

Select Batch:

Ficha de datos (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Muscarinic Acetylcholine Receptor M2 Antibody (YA2610) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Productos vistos recientemente:

Nombre del producto

Requested Quantity *

Nombre del solicitante *

Saludo

Direcciòn del E-mail *

Número de teléfono *

Department

Nombre de la Organizaciòn *

City

Provincia

Country or Region *

Observaciones

Nombre del producto

Lot #

Nombre del solicitante *

Country or Region *

Direcciòn del E-mail *

Observaciones

Name
E-mail *

	Sorry, but the email address you supplied was invalid.

Muscarinic Acetylcholine Receptor M2 Antibody (YA2610)

Muscarinic Acetylcholine Receptor M2 Antibody (YA2610) Recomendaciones destacadas:

Product Detail

Verification Image

Background

Descripciòn

Muscarinic Acetylcholine Receptor M2 Antibody (YA2610) Related Classifications

Productos vistos recientemente:

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report