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CRABP2 Antibody (YA1652)

(Synonyms: Cellular retinoic acid-binding protein II; CRABP-II)
Customer Review

Based on 1 Customer Validation

CRABP2 Antibody (YA1652) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CRABP2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for CRABP2 Antibody (YA1652)
  • Experimental Validation Results for CRABP2 Antibody (YA1652)
  • Experimental Validation Results for CRABP2 Antibody (YA1652)
  • CRABP2 Antibody (YA1652) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

CRABP2 Antibody (YA1652) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CRABP2.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 16 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 16 kDa
Species Reactivity Database

Entrez Gene: 1382 Human

SwissProt: P29373 Human

Immunogen

Recombinant protein of human CRABP2

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104004

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CRABP2 Antibody (YA1652)
    Western blot analysis of extracts from MCF-7(lane 2(20ug) , HT-29(lane 3(20ug) and HEK293(lane 4(20ug) using CRABP2 Antibody (HY-P81907) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CRABP2 Antibody (YA1652)
    Immunocytochemistry analysis of Hela cells labeling CRABP2 with CRABP2 Antibody (HY-P81907) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CRABP2 Antibody (HY-P81907) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CRABP2 Antibody (YA1652)
    Immunocytochemistry analysis of MCF-7 cells labeling CRABP2 with CRABP2 Antibody (HY-P81907) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CRABP2 Antibody (HY-P81907) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    CRABP2 transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors

  • Subcellular Localization

    Cytoplasm; Endoplasmic reticulum; Nucleus

  • Expression


    Induction:By retinoic acid

  • Subunit

    Interacts with RXR and RARA (By similarity). Interacts with importin alpha

  • SwissProt ID

    P29373

  • Gene ID
    1382 [NCBI]
  • Synonyms

    Cellular retinoic acid-binding protein II; CRABP-II

  • Research Field

    Signal Transduction

CRABP2 Antibody (YA1652) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20ug) , HT-29(lane 3(20ug) and HEK293(lane 4(20ug) using CRABP2 Antibody (HY-P81907) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling CRABP2 with CRABP2 Antibody (HY-P81907) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CRABP2 Antibody (HY-P81907) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling CRABP2 with CRABP2 Antibody (HY-P81907) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CRABP2 Antibody (HY-P81907) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CRABP2 Antibody (YA1652) Product Image
    1/16
  • Experimental Validation Results for CRABP2 Antibody (YA1652)
  • Experimental Validation Results for CRABP2 Antibody (YA1652)
  • Experimental Validation Results for CRABP2 Antibody (YA1652)
  • CRABP2 Antibody (YA1652) Product Image
  • Experimental Validation Results for CRABP2 Antibody (YA1652)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7(lane 2(20ug) , HT-29(lane 3(20ug) and HEK293(lane 4(20ug) using CRABP2 Antibody (HY-P81907) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CRABP2 Antibody (YA1652)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling CRABP2 with CRABP2 Antibody (HY-P81907) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CRABP2 Antibody (HY-P81907) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CRABP2 Antibody (YA1652)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling CRABP2 with CRABP2 Antibody (HY-P81907) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CRABP2 Antibody (HY-P81907) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CRABP2 Antibody (YA1652)
  • Experimental Validation Results for CRABP2 Antibody (YA1652)
  • Experimental Validation Results for CRABP2 Antibody (YA1652)
  • CRABP2 Antibody (YA1652) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7(lane 2(20ug) , HT-29(lane 3(20ug) and HEK293(lane 4(20ug) using CRABP2 Antibody (HY-P81907) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CRABP2 Antibody (YA1652) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling CRABP2 with CRABP2 Antibody (HY-P81907) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CRABP2 Antibody (HY-P81907) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CRABP2 Antibody (YA1652) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling CRABP2 with CRABP2 Antibody (HY-P81907) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with CRABP2 Antibody (HY-P81907) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CRABP2 Antibody (YA1652) Product Image WB
  • CRABP2 Antibody (YA1652) Product Image ICC
  • CRABP2 Antibody (YA1652) Product Image ICC