Glucose 6 Phosphate Dehydrogenase Antibody (YA4869)

Cat. No.: HY-P85177: Data Sheet User Guide for Antibodies
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Glucose 6 Phosphate Dehydrogenase Antibody (YA4869) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Glucose 6 Phosphate Dehydrogenase.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Glucose 6 Phosphate Dehydrogenase Antibody (YA4869) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to Glucose 6 Phosphate Dehydrogenase.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 59 kDa;

Species Reactivity

Human

SwissProt ID

P11413

Gene ID

2539 [NCBI]

Immunogen

Purified recombinant fragment of human G6PD expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis

Subcellular Localization：Cytoplasm, cytosol; Membrane; Peripheral membrane protein

Expression：
Tissue_specificity:The long subtype is found in lymphoblasts, granulocytes, and sperm.

Isoforms & Post-Translational Modification：P11413 has 3 isomers: P11413-1: 59257 Da (predicted); P11413-2: 63827 Da (predicted); P11413-3: 62468 Da (predicted).
Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity

Subunit：Homotetramer; dimer of dimers (PubMed:10745013, PubMed:15858258, PubMed:24769394, PubMed:38066190). Interacts with SIRT2; the interaction is enhanced by H(2)O(2) treatment (PubMed:24769394). Forms a ternary complex with ALDOB and TP53; this interaction is direct. ALDOB stabilizes the complex inhibiting G6PD activity and keeping oxidative pentose phosphate metabolism in check

Synonyms

G6PD; Glucose-6-phosphate 1-dehydrogenase; G6PD

Documentation

User Guide for Antibodies (1077 KB)