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Factor VIII Antibody

(Synonyms: F8; F8C; Coagulation factor VIII; Antihemophilic factor; AHF; Procoagulant component)
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Based on 1 Customer Validation

Factor VIII Antibody (YA4848) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Factor VIII.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Factor VIII Antibody
  • Factor VIII Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:100-1:300 1:50-1:200 1:10000

Product Details

Beschreibung

Factor VIII Antibody (YA4848) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Factor VIII.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse
  • Calculated Molecular Weight Predicted band size: 267 kDa;
Immunogen

Purified recombinant C-terminal fragment of Factor VIII expressed in E. Coli.

Sensitivity

Endogenous

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Versand

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Factor VIII Antibody
    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2) using Factor VIII antibody. Proteins were transferred onto a 0.22 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody β-actin Antibody (HRP) (HY-P80993) (1:10000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Factor VIII (FVIII) is an essential blood coagulation protein, is a key component of the fluid phase blood coagulation system. Factor VIII is also a large procoagulant glycoprotein and a metal ion-dependent heterodimer that circulates in plasma in a noncovalent complex with von Willebrand factor. Factor VIII absence causes a severe bleeding disorder.

  • Subcellular Localization

    Secreted, extracellular space

  • Isoforms & Post-Translational Modification

    P00451 has 2 isomers: P00451-1: 267009 Da (predicted); P00451-2: 24641 Da (predicted).
    Sulfation on Tyr-1699 is essential for binding vWF;Proteolytically cleaved by cathepsin CTSG to produce a partially activated form

  • Subunit

    Interacts with VWF/vWF. vWF binding is essential for the stabilization of F8 in circulation

  • SwissProt ID

    P00451

  • Gene ID
    2157 [NCBI]
  • Synonyms

    F8; F8C; Coagulation factor VIII; Antihemophilic factor; AHF; Procoagulant component

[1]. Mazurkiewicz-Pisarek A, et al. The factor VIII protein and its function. Acta Biochim Pol. 2016;63(1):11-16. [Content Brief]

[2]. Fay PJ, Jenkins PV. Mutating factor VIII: lessons from structure to function. Blood Rev. 2005 Jan;19(1):15-27. [Content Brief]

[3]. Hoyer LW. Molecular pathology and immunology of factor VIII (hemophilia A and factor VIII inhibitors). Hum Pathol. 1987 Feb;18(2):153-61. [Content Brief]

Factor VIII Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2) using Factor VIII antibody. Proteins were transferred onto a 0.22 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody β-actin Antibody (HRP) (HY-P80993) (1:10000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Factor VIII Antibody Product Image
    1/16
  • Experimental Validation Results for Factor VIII Antibody
  • Factor VIII Antibody Product Image
  • Experimental Validation Results for Factor VIII Antibody
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2) using Factor VIII antibody. Proteins were transferred onto a 0.22 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody β-actin Antibody (HRP) (HY-P80993) (1:10000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Factor VIII Antibody
  • Factor VIII Antibody Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from HepG2 (lane 2) using Factor VIII antibody. Proteins were transferred onto a 0.22 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody β-actin Antibody (HRP) (HY-P80993) (1:10000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Factor VIII Antibody Product Image WB