Factor VIII Antibody

Cat. No.: HY-P85156: Data Sheet User Guide for Antibodies
FAQs
Technical Support

Factor VIII Antibody (YA4848) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Factor VIII.

For research use only. We do not sell to patients.

Size	Price	Stock
20 μL		Ask For Quote & Lead Time
50 μL		Ask For Quote & Lead Time
100 μL		Ask For Quote & Lead Time

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Factor VIII Antibody (YA4848) is a Rabbit-derived and non-conjugated monoclonal antibody, targeting to Factor VIII.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 267 kDa;

Species Reactivity

Human, Mouse

SwissProt ID

P00451

Gene ID

2157 [NCBI]

Immunogen

Purified recombinant C-terminal fragment of Factor VIII expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:300
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	Purity	affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa

Subcellular Localization：Secreted, extracellular space

Isoforms & Post-Translational Modification：P00451 has 2 isomers: P00451-1: 267009 Da (predicted); P00451-2: 24641 Da (predicted).
Sulfation on Tyr-1699 is essential for binding vWF;Proteolytically cleaved by cathepsin CTSG to produce a partially activated form

Subunit：Interacts with VWF/vWF. vWF binding is essential for the stabilization of F8 in circulation

Synonyms

F8; F8C; Coagulation factor VIII; Antihemophilic factor; AHF; Procoagulant component

Documentation

User Guide for Antibodies (1077 KB)