PEG10 Antibody (YA5015)

Cat. No.: HY-P85323: Data Sheet User Guide for Antibodies
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PEG10 Antibody (YA5015) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to PEG10.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

PEG10 Antibody (YA5015) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to PEG10.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 80 kDa;

Observed band size: 37,80 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q86TG7

Gene ID

23089 [NCBI]

Immunogen

Purified recombinant fragment of human PEG10 expressed in E. Coli. The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200

Sensitivity	Endogenous	Purity	Ascitic Fluid
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in purified antibody in PBS with 0.05% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Retrotransposon-derived protein that binds its own mRNA and self-assembles into virion-like capsids (PubMed:34413232). Forms virion-like extracellular vesicles that encapsulate their own mRNA and are released from cells, enabling intercellular transfer of PEG10 mRNA (PubMed:34413232). Binds its own mRNA in the 5'-UTR region, in the region near the boundary between the nucleocapsid (NC) and protease (PRO) coding sequences and in the beginning of the 3'-UTR region (PubMed:34413232). Involved in placenta formation: required for trophoblast stem cells differentiation (By similarity). Involved at the immediate early stage of adipocyte differentiation (By similarity). Overexpressed in many cancers and enhances tumor progression: promotes cell proliferation by driving cell cycle progression from G0/G1 (PubMed:12810624, PubMed:16423995, PubMed:26235627, PubMed:28193232). Enhances cancer progression by inhibiting the TGF-beta signaling, possibly via interaction with the TGF-beta receptor ACVRL1 (PubMed:15611116, PubMed:26235627, PubMed:30094509). May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter; additional evidences are however required to confirm this result (By similarity)

Subcellular Localization：Extracellular vesicle membrane; Cytoplasm; Nucleus

Expression：
Tissue_specificity:It is expressed in the cytotrophoblast of the placenta, but not in the overlying syncytiotrophoblast. It is expressed in prostate and breast cancer, but not in normal breast and prostate epithelial cells. It is expressed in the Hep-G2 cell line (protein level) . It is expressed in the brain, liver, spleen, kidney, thymus, lung, ovary, testis, reactive lymph nodes, skeletal muscle, adipose tissue, and placenta. It is expressed in pancreatic cancer and hepatocellular carcinoma (HCC) .

Induction:Expression is directly regulated by E2F1 and E2F4, which bind to its promoter and direct its expression (PubMed:17050006, PubMed:18625225, PubMed:28193232, PubMed:28992046) . Up-regulated by MYC (PubMed:16423995) . Strongly overepressed in a number of tumors, such has hepatocellular carcinoma (HCC) , pancreatic or neuroendocrine prostate cancers (PubMed:20362226, PubMed:26235627, PubMed:28193232)

Isoforms & Post-Translational Modification：Q86TG7 has 5 isomers: Q86TG7-1: 80173 Da (predicted); Q86TG7-2: 36966 Da (predicted); Q86TG7-3: 40490 Da (predicted); Q86TG7-4: 88281 Da (predicted); Q86TG7-5: 45074 Da (predicted).
Undergoes proteolytic cleavage

Subunit：Homooligomer; homooligomerizes into virion-like capsids (By similarity). Interacts with ACVRL1 (PubMed:15611116). Interacts with SIAH1 and SIAH2 (PubMed:12810624)

Synonyms

PEG10; EDR; KIAA1051; MAR2; MART2; MEF3L1; RGAG3; Retrotransposon-derived protein PEG10; Embryonal carcinoma differentiation-regulated protein; Mammalian retrotransposon-derived protein 2; Myelin expression factor 3-like protein 1; MEF3-lik

Documentation

User Guide for Antibodies (1077 KB)