Myosin Heavy Chain Smooth Muscle Antibody (YA5655)

Cat. No.: HY-P85963: Data Sheet User Guide for Antibodies
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Myosin Heavy Chain Smooth Muscle Antibody (YA5655) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Myosin Heavy Chain Smooth Muscle.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Myosin Heavy Chain Smooth Muscle Antibody (YA5655) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Myosin Heavy Chain Smooth Muscle.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 227 kDa;

Observed band size: 227 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P35749

Gene ID

4629 [NCBI]

Immunogen

Synthesized peptide derived from human Myosin Heavy Chain, Smooth Muscle AA range: 300-400

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1000
WB WB: Western Blot	1:500-2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:1000-5000

Purity	Protein G	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Muscle contraction

Subcellular Localization：Melanosome

Expression：
Tissue_specificity:Smooth muscle; expressed in the umbilical artery, bladder, esophagus, and trachea. Isotype 1 is mainly found in slowly contracting tonifying muscles.

Isoforms & Post-Translational Modification：P35749 has 4 isomers: P35749-1: 227339 Da (predicted); P35749-2: 228090 Da (predicted); P35749-3: 224328 Da (predicted); P35749-4: 223577 Da (predicted).

Subunit：Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2)

Synonyms

Myosin-11; Myosin heavy chain 11; Myosin heavy chain, smooth muscle isoform; SMMHC;

Documentation

User Guide for Antibodies (1077 KB)