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Phospho-alpha Synuclein (Ser129) Antibody (YA1484)

(Synonyms: SNCA; NACP; PARK1; Alpha-synuclein; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP)
Customer Review

Based on 1 Customer Validation

Phospho-alpha Synuclein (Ser129) Antibody (YA1484) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-alpha Synuclein (Ser129).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Phospho-alpha Synuclein (Ser129) Antibody (YA1484)
  • Experimental Validation Results for Phospho-alpha Synuclein (Ser129) Antibody (YA1484)
  • Phospho-alpha Synuclein (Ser129) Antibody (YA1484) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Phospho-alpha Synuclein (Ser129) Antibody (YA1484) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-alpha Synuclein (Ser129).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 18 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 14 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Phospho-alpha Synuclein (S129) EMP-pS-EEG.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3103845

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Phospho-alpha Synuclein (Ser129) Antibody (YA1484)
    Immunocytochemistry analysis of C6 cells labeling Phospho-alpha Synuclein (Ser129)Antibody (HY-P81739) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-alpha Synuclein (Ser129)Antibody (HY-P81739) at 1/50 dilution in BSA for Immunol Staining at 4 ℃overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-alpha Synuclein (Ser129) Antibody (YA1484)
    Immunocytochemistry analysis of Hela cells labeling Phospho-alpha Synuclein (Ser129)Antibody (HY-P81739) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-alpha Synuclein (Ser129)Antibody (HY-P81739) at 1/50 dilution in BSA for Immunol Staining at 4 ℃overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    alpha Synuclein is a Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity[1][2][3][4].

  • Subcellular Localization

    Cytoplasm; Membrane; Nucleus; Synapse; Secreted; Cell projection, axon

  • Expression


    Tissue_specificity:It is highly expressed at presynaptic terminals in the central nervous system. It is mainly expressed in brain tissue.

  • Isoforms & Post-Translational Modification

    P37840 has 3 isomers: P37840-1: 14460 Da (predicted); P37840-2: 11372 Da (predicted); P37840-3: 13109 Da (predicted).
    Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress;Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers;Ubiquitinated. The predominant conjugate is the diubiquitinated form;Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure

  • Subunit

    Soluble monomer. Homotetramer (PubMed:21841800). A dynamic intracellular population of tetramers and monomers coexists normally and the tetramer plays an essential role in maintaining homeostasis (PubMed:21841800).

  • SwissProt ID

    P37840

  • Gene ID
    6622 [NCBI]
  • Synonyms

    SNCA; NACP; PARK1; Alpha-synuclein; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; NACP

  • Research Field

    Neuroscience

Phospho-alpha Synuclein (Ser129) Antibody (YA1484) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Phospho-alpha Synuclein (Ser129)Antibody (HY-P81739) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-alpha Synuclein (Ser129)Antibody (HY-P81739) at 1/50 dilution in BSA for Immunol Staining at 4 ℃overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Phospho-alpha Synuclein (Ser129)Antibody (HY-P81739) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-alpha Synuclein (Ser129)Antibody (HY-P81739) at 1/50 dilution in BSA for Immunol Staining at 4 ℃overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-alpha Synuclein (Ser129) Antibody (YA1484) Product Image
    1/16
  • Experimental Validation Results for Phospho-alpha Synuclein (Ser129) Antibody (YA1484)
  • Experimental Validation Results for Phospho-alpha Synuclein (Ser129) Antibody (YA1484)
  • Phospho-alpha Synuclein (Ser129) Antibody (YA1484) Product Image
  • Experimental Validation Results for Phospho-alpha Synuclein (Ser129) Antibody (YA1484)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Phospho-alpha Synuclein (Ser129)Antibody (HY-P81739) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-alpha Synuclein (Ser129)Antibody (HY-P81739) at 1/50 dilution in BSA for Immunol Staining at 4 ℃overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-alpha Synuclein (Ser129) Antibody (YA1484)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Phospho-alpha Synuclein (Ser129)Antibody (HY-P81739) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-alpha Synuclein (Ser129)Antibody (HY-P81739) at 1/50 dilution in BSA for Immunol Staining at 4 ℃overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Phospho-alpha Synuclein (Ser129) Antibody (YA1484)
  • Experimental Validation Results for Phospho-alpha Synuclein (Ser129) Antibody (YA1484)
  • Phospho-alpha Synuclein (Ser129) Antibody (YA1484) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of C6 cells labeling Phospho-alpha Synuclein (Ser129)Antibody (HY-P81739) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-alpha Synuclein (Ser129)Antibody (HY-P81739) at 1/50 dilution in BSA for Immunol Staining at 4 ℃overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-alpha Synuclein (Ser129) Antibody (YA1484) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Phospho-alpha Synuclein (Ser129)Antibody (HY-P81739) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Phospho-alpha Synuclein (Ser129)Antibody (HY-P81739) at 1/50 dilution in BSA for Immunol Staining at 4 ℃overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Phospho-alpha Synuclein (Ser129) Antibody (YA1484) Product Image ICC
  • Phospho-alpha Synuclein (Ser129) Antibody (YA1484) Product Image ICC