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TOMM40 Antibody (YA6809)

(Synonyms: C19orf1 antibody; D19S1177E antibody; Haymaker protein antibody; Mitochondrial import receptor subunit TOM40 homolog antibody; Mitochondrial outer membrane protein antibody; Mitochondrial outer membrane protein TOM40 antibody; p38.5 antibody; PER EC1 antibody; PEREC1 antibody; Probable mitochondrial import receptor subunit TOM40 homolog antibody; C19orf1 antibody; D19S1177E antibody; Haymaker protein antibody; Mitochondrial import receptor subunit TOM40 homolog antibody)
Customer Review

Based on 1 Customer Validation

TOMM40 Antibody (YA6809) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TOMM40.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P mIHC
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • TOMM40 Antibody (YA6809) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000 1:200-1:1000 1:100 1:1000 1-2μg/sample

Product Details

Description

TOMM40 Antibody (YA6809) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TOMM40.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 38 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 38 kDa
Immunogen

Recombinant protein within human TOMM40 aa 1-361/361

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719264

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using TOMM40 Antibody (HY-P87116) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    Immunohistochemical analysis of paraffin-embedded human Liver Cancer tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

Background

  • Function

    TOMM40 is a Channel-forming protein essential for import of protein precursors into mitochondria. Plays a role in the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) by forming a complex with BCAP31 and mediating the translocation of Complex I components from the cytosol to the mitochondria[1][2].

  • Subcellular Localization

    Mitochondrion outer membrane

  • Isoforms & Post-Translational Modification

    O96008 has two isomers: O96008-1: 37893 Da (predicted); O96008-2: 34441 Da (predicted).

  • Subunit

    Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70)

  • SwissProt ID

    O96008

  • Gene ID
    10452 [NCBI]
  • Synonyms

    C19orf1 antibody; D19S1177E antibody; Haymaker protein antibody; Mitochondrial import receptor subunit TOM40 homolog antibody; Mitochondrial outer membrane protein antibody; Mitochondrial outer membrane protein TOM40 antibody; p38.5 antibody; PER EC1 antibody; PEREC1 antibody; Probable mitochondrial import receptor subunit TOM40 homolog antibody; C19orf1 antibody; D19S1177E antibody; Haymaker protein antibody; Mitochondrial import receptor subunit TOM40 homolog antibody

TOMM40 Antibody (YA6809) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using TOMM40 Antibody (HY-P87116) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver Cancer tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • 1/16

    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • TOMM40 Antibody (YA6809) Product Image
    1/16
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • TOMM40 Antibody (YA6809) Product Image
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using TOMM40 Antibody (HY-P87116) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Liver Cancer tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
    1/1
    MedchemExpress Validation
     Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • Experimental Validation Results for TOMM40 Antibody (YA6809)
  • TOMM40 Antibody (YA6809) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 1(20μg)) 、HeLa (lane 2(20μg)) 、NIH-3T3 (lane 3(20μg)) and PC-12 (lane 4(20μg)) using TOMM40 Antibody (HY-P87116) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • TOMM40 Antibody (YA6809) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TOMM40 Antibody (YA6809) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TOMM40 Antibody (YA6809) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Esophageal Carcinoma tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TOMM40 Antibody (YA6809) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Gastric Cancer tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TOMM40 Antibody (YA6809) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Ovarian Cancer‌ tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TOMM40 Antibody (YA6809) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Liver Cancer tissue using TOMM40 antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P87116, 1:200 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TOMM40 Antibody (YA6809) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • TOMM40 Antibody (YA6809) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • TOMM40 Antibody (YA6809) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Colon cancer‌ tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • TOMM40 Antibody (YA6809) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • TOMM40 Antibody (YA6809) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • TOMM40 Antibody (YA6809) Product Image
    MedchemExpress Validation

    Tyramide signaling amplification based immunofluorescence was performed on paraffin-embedded human Gastric Cancer tissue using TOMM40 antibody. Antigen retrieval was performed in EDTA buffer pH 9.0 (95 °C, 20 min) followed by cooling to RT. Then incubated with primary antibody (HY-P87116, 1:400 dilution) at room temperature for 60 minutes and HRP conjugated secondary antibody for 10 minutes. Fluorescence was then developed with Vari Fluor 532 TSA (200×)（HY-D1832）. The tissues were counterstained with DAPI and mounted with Anti-fade mounting medium.

  • TOMM40 Antibody (YA6809) Product Image WB
  • TOMM40 Antibody (YA6809) Product Image IHC-P
  • TOMM40 Antibody (YA6809) Product Image IHC-P
  • TOMM40 Antibody (YA6809) Product Image IHC-P
  • TOMM40 Antibody (YA6809) Product Image IHC-P
  • TOMM40 Antibody (YA6809) Product Image IHC-P
  • TOMM40 Antibody (YA6809) Product Image IHC-P
  • TOMM40 Antibody (YA6809) Product Image mIHC
  • TOMM40 Antibody (YA6809) Product Image mIHC
  • TOMM40 Antibody (YA6809) Product Image mIHC
  • TOMM40 Antibody (YA6809) Product Image mIHC
  • TOMM40 Antibody (YA6809) Product Image mIHC
  • TOMM40 Antibody (YA6809) Product Image mIHC