Copy Link Print Information

GCDFP-15 Antibody (YA2242)

(Synonyms: BRST2; GCDGP15; gp17; GPIP4; PIP; Prolactin induced protein; SABP; Secretory actin binding protein)
Customer Review

Based on 1 Customer Validation

GCDFP-15 Antibody (YA2242) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GCDFP-15.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for GCDFP-15 Antibody (YA2242)
  • Experimental Validation Results for GCDFP-15 Antibody (YA2242)
  • Experimental Validation Results for GCDFP-15 Antibody (YA2242)
  • GCDFP-15 Antibody (YA2242) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50

Product Details

Description

GCDFP-15 Antibody (YA2242) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GCDFP-15.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 17 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 17 kDa
Species Reactivity Database

Entrez Gene: 5304 Human

SwissProt: P12273 Human

Immunogen

A synthesized peptide derived from human GCDFP 15 aa100-146/146.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104604

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for GCDFP-15 Antibody (YA2242)
    Western blot analysis was performed on extracts from T47D (lane 1, 15 μg) using GCDFP 15 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for GCDFP-15 Antibody (YA2242)
    Immunocytochemistry analysis of MCF-7 cells labeling GCDFP-15 with GCDFP-15 antibody (HY-P82497) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with GCDFP-15 antibody (HY-P82497) at 1/50 dilution in BSA for Immunol Staining at 4℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for GCDFP-15 Antibody (YA2242)
    Immunocytochemistry analysis of HeLa cells labeling GCDFP-15 with GCDFP-15 antibody (HY-P82479) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with GCDFP-15 antibody (HY-P82497) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Gross cystic disease fluid protein-15 (GCDFP-15; prolactin-inducible protein, PIP) is a secreted glycoprotein expressed by several exocrine epithelia and implicated in epithelial biology and immune modulation. GCDFP-15 as an upstream inhibitor that links impaired vascular and immune programs to delayed healing in diabetes and nominate GCDFP-15 as a therapeutic target to restore reparative signalling[1].

  • Subcellular Localization

    Secreted

  • Expression


    Tissue_specificity:It is expressed in breast pathology and in various exocrine tissues (such as lacrimal glands, salivary glands, and sweat glands) .

    Induction:By prolactin and androgen; inhibited by estrogen

  • Subunit

    Monomer. Interacts with AZGP1

  • SwissProt ID

    P12273

  • Gene ID
    5304 [NCBI]
  • Synonyms

    BRST2; GCDGP15; gp17; GPIP4; PIP; Prolactin induced protein; SABP; Secretory actin binding protein

  • Research Field

    Tags & Cell Markers

References

[1]. Zhang C, et al. GCDFP-15 impairs diabetic cutaneous wound repair in mice by suppressing the PI3K-AKT-mTOR-STAT3 pathway. Arch Physiol Biochem. 2026 Apr 20:1-13.

GCDFP-15 Antibody (YA2242) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from T47D (lane 1, 15 μg) using GCDFP 15 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling GCDFP-15 with GCDFP-15 antibody (HY-P82497) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with GCDFP-15 antibody (HY-P82497) at 1/50 dilution in BSA for Immunol Staining at 4℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling GCDFP-15 with GCDFP-15 antibody (HY-P82479) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with GCDFP-15 antibody (HY-P82497) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • GCDFP-15 Antibody (YA2242) Product Image
    1/16
  • Experimental Validation Results for GCDFP-15 Antibody (YA2242)
  • Experimental Validation Results for GCDFP-15 Antibody (YA2242)
  • Experimental Validation Results for GCDFP-15 Antibody (YA2242)
  • GCDFP-15 Antibody (YA2242) Product Image
  • Experimental Validation Results for GCDFP-15 Antibody (YA2242)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from T47D (lane 1, 15 μg) using GCDFP 15 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for GCDFP-15 Antibody (YA2242)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling GCDFP-15 with GCDFP-15 antibody (HY-P82497) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with GCDFP-15 antibody (HY-P82497) at 1/50 dilution in BSA for Immunol Staining at 4℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for GCDFP-15 Antibody (YA2242)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling GCDFP-15 with GCDFP-15 antibody (HY-P82479) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with GCDFP-15 antibody (HY-P82497) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for GCDFP-15 Antibody (YA2242)
  • Experimental Validation Results for GCDFP-15 Antibody (YA2242)
  • Experimental Validation Results for GCDFP-15 Antibody (YA2242)
  • GCDFP-15 Antibody (YA2242) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from T47D (lane 1, 15 μg) using GCDFP 15 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P80137, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • GCDFP-15 Antibody (YA2242) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling GCDFP-15 with GCDFP-15 antibody (HY-P82497) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with GCDFP-15 antibody (HY-P82497) at 1/50 dilution in BSA for Immunol Staining at 4℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • GCDFP-15 Antibody (YA2242) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling GCDFP-15 with GCDFP-15 antibody (HY-P82479) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with GCDFP-15 antibody (HY-P82497) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • GCDFP-15 Antibody (YA2242) Product Image WB
  • GCDFP-15 Antibody (YA2242) Product Image ICC
  • GCDFP-15 Antibody (YA2242) Product Image ICC