Sm-D2 Antibody
(Synonyms: SNRPD1, SNRPD2, Small nuclear ribonucleoprotein Sm D2, Sm-D2, snRNP core protein D2)Sm-D2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Sm-D2.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
Sm-D2 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Sm-D2.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 13 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 12; 13 kDa
Purified recombinant protein of human Sm-D2
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Envío
Shipping with blue ice.
Background
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Function
Sm-D2 plays a role in pre-mRNA splicing as a core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Component of both the pre-catalytic spliceosome B complex and activated spliceosome C complexes. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs[1][2][3][4][5][6][7][8][9][10][11].
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Subcellular Localization
Cytoplasm, cytosol; Nucleus
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Isoforms & Post-Translational Modification
Sm-D2 has 2 isoforms, P62316-1: amino acid length is 118, molecular weight is 13527 Da (predicted); P62316-2: amino acid length is 108, molecular weight is 12399 Da (predicted).
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Subunit
Core component of the spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome.
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SwissProt ID
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Synonyms
SNRPD1, SNRPD2, Small nuclear ribonucleoprotein Sm D2, Sm-D2, snRNP core protein D2
Documentación
[1]. Jurica MS, et al. Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. RNA. 2002 Apr;8(4):426-39. [Content Brief]
[2]. Chari A, et al. An assembly chaperone collaborates with the SMN complex to generate spliceosomal SnRNPs. Cell. 2008 Oct 31;135(3):497-509. [Content Brief]
[3]. Pomeranz Krummel DA, et al. Crystal structure of human spliceosomal U1 snRNP at 5.5 A resolution. Nature. 2009 Mar 26;458(7237):475-80. [Content Brief]
[4]. Grimm C, et al. Structural basis of assembly chaperone- mediated snRNP formation. Mol Cell. 2013 Feb 21;49(4):692-703. [Content Brief]
[5]. Kondo Y, et al. Crystal structure of human U1 snRNP, a small nuclear ribonucleoprotein particle, reveals the mechanism of 5' splice site recognition. Elife. 2015 Jan 2;4:. [Content Brief]
[6]. Agafonov DE, et al. Molecular architecture of the human U4/U6.U5 tri-snRNP. Science. 2016 Mar 25;351(6280):1416-20. [Content Brief]
[7]. Bertram K, et al. Cryo-EM structure of a human spliceosome activated for step 2 of splicing. Nature. 2017 Feb 16;542(7641):318-323. [Content Brief]
[8]. Zhang X, et al. An Atomic Structure of the Human Spliceosome. Cell. 2017 May 18;169(5):918-929.e14. [Content Brief]
[9]. Bertram K, et al. Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation. Cell. 2017 Aug 10;170(4):701-713.e11. [Content Brief]
[10]. Zhang Z, et al. Molecular architecture of the human 17S U2 snRNP. Nature. 2020 Jul;583(7815):310-313. [Content Brief]
[11]. Will CL, et al. The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome. RNA. 2004 Jun;10(6):929-41. [Content Brief]
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