Phospho-GRLF1 (Tyr1105) Antibody
(Synonyms: GRF1, GRLF1, KIAA1722, P190A, p190ARHOGAP, ARHGAP35, Rho GTPase-activating protein 35, Glucocorticoid receptor DNA-binding factor 1, Glucocorticoid receptor repression factor 1, Rho GAP p190A, GRF-1, p190-A)Phospho-GRLF1 (Tyr1105) Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Phospho-GRLF1 (Tyr1105).
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
Phospho-GRLF1 (Tyr1105) Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to Phospho-GRLF1 (Tyr1105).
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 190 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 170 kDa
Synthetic phosphopeptide corresponding to residues surrounding Y1105 of human GRLF1 protein.
Endogenous
affinity purified.
Non-conjugated
Phosphorylated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
GRLF1 is a Rho GTPase-activating protein (GAP). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity. This binding is inhibited by phosphorylation by PRKCA. Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (By similarity). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (By similarity). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (By similarity). Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (By similarity). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (By similarity). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences[1][2][3].
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Subcellular Localization
Cytoplasm, cytoskeleton, cilium basal body; Cytoplasm; Nucleus; Cell membrane
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Expression
Tissue_Specificity: Detected in neutrophils (at protein level). -
Isoforms & Post-Translational Modification
Phospho-GRLF1 has an amino acid length of 1499, molecular weight is 170514 Da.
Phosphorylation of Tyr-1105 by PTK6 promotes the association with RASA1, inactivating RHOA while activating RAS. Phosphorylation at Tyr-308 by PDGFRA inhibits binding to GTF2I. Phosphorylated by PRKCA at Ser-1221 and Thr-1226, induces relocalization from the cytoplasm to regions of plasma membrane ruffling and prevents the binding and substrate specificity regulation by phospholipids. -
Subunit
Interacts with RASA1 (By similarity).
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SwissProt ID
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Synonyms
GRF1, GRLF1, KIAA1722, P190A, p190ARHOGAP, ARHGAP35, Rho GTPase-activating protein 35, Glucocorticoid receptor DNA-binding factor 1, Glucocorticoid receptor repression factor 1, Rho GAP p190A, GRF-1, p190-A
Documentation
References
[1]. Lévay M, et al. Regulation of the substrate preference of p190RhoGAP by protein kinase C-mediated phosphorylation of a phospholipid binding site. Biochemistry. 2009 Sep 15;48(36):8615-23. [Content Brief]
[2]. Stiegler AL, et al. p190RhoGAP proteins contain pseudoGTPase domains. Nat Commun. 2017 Sep 11;8(1):506. [Content Brief]
[3]. LeClerc S, et al. Molecular cloning and characterization of a factor that binds the human glucocorticoid receptor gene and represses its expression. J Biol Chem. 1991 Sep 15;266(26):17333-40. [Content Brief]
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