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  4. EFEMP1 Antibody (YA2883)

EFEMP1 Antibody (YA2883) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EFEMP1.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay
  • Product Detail

  • Verification Image

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  • Descripciòn

Descripciòn

EFEMP1 Antibody (YA2883) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EFEMP1.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Peso molecular
Predicted band size: 55 kDa;
Observed band size: 55 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Recombinant protein of human EFEMP1/Fibulin-3 aa18-493.

Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Pureza Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image
WB
  • Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using EFEMP1 (HY-P83138) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The pri-mary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat An-ti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function:Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth
Subcellular Localization:Secreted, extracellular space, extracellular matrix
Expression:
Tissue_specificity:In the eye, associated with photoreceptor outer and inner segment regions, the nerve fiber layer, outer nuclear layer and inner and outer plexiform layers of the retina
Isoforms & Post-Translational Modification:Q12805 has 5 isomers: Q12805-1: 54641 Da (predicted); Q12805-2: 53722 Da (predicted); Q12805-3: 54583 Da (predicted); Q12805-4: 54553 Da (predicted); Q12805-5: 39195 Da (predicted).
Subunit:Interacts with ECM1. Interacts with TIMP3
RRID
Database
Research Field

-

Synonyms
DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; FBLN3; FIBL-3; Fibulin-3
Documentación

EFEMP1 Antibody (YA2883) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

Nombre del producto:
EFEMP1 Antibody (YA2883)
Cat. No.:
HY-P83138
Cantidad:
MCE Japan Authorized Agent: