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EFEMP1 Antibody (YA2883)

(Synonyms: DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; FBLN3; FIBL-3; Fibulin-3)
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Based on 1 Customer Validation

EFEMP1 Antibody (YA2883) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EFEMP1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for EFEMP1 Antibody (YA2883)
  • EFEMP1 Antibody (YA2883) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:20

Product Details

Description

EFEMP1 Antibody (YA2883) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to EFEMP1.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 55 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 55 kDa
Species Reactivity Database

Entrez Gene: 2202 Human

SwissProt: Q12805 Human

Immunogen

Recombinant protein of human EFEMP1/Fibulin-3 aa18-493.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105253

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EFEMP1 Antibody (YA2883)
    Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using EFEMP1 (HY-P83138) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The pri-mary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat An-ti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    EFEMP1 binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix

  • Expression


    Tissue_specificity:In the eye, associated with photoreceptor outer and inner segment regions, the nerve fiber layer, outer nuclear layer and inner and outer plexiform layers of the retina

  • Isoforms & Post-Translational Modification

    Q12805 has 5 isomers: Q12805-1: 54641 Da (predicted); Q12805-2: 53722 Da (predicted); Q12805-3: 54583 Da (predicted); Q12805-4: 54553 Da (predicted); Q12805-5: 39195 Da (predicted).

  • Subunit

    Interacts with ECM1. Interacts with TIMP3

  • SwissProt ID

    Q12805

  • Gene ID
    2202 [NCBI]
  • Synonyms

    DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; FBLN3; FIBL-3; Fibulin-3

  • Research Field

    -

EFEMP1 Antibody (YA2883) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using EFEMP1 (HY-P83138) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The pri-mary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat An-ti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • EFEMP1 Antibody (YA2883) Product Image
    1/16
  • Experimental Validation Results for EFEMP1 Antibody (YA2883)
  • EFEMP1 Antibody (YA2883) Product Image
  • Experimental Validation Results for EFEMP1 Antibody (YA2883)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using EFEMP1 (HY-P83138) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The pri-mary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat An-ti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for EFEMP1 Antibody (YA2883)
  • EFEMP1 Antibody (YA2883) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg) and Hela (lane 3(40μg) using EFEMP1 (HY-P83138) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The pri-mary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat An-ti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • EFEMP1 Antibody (YA2883) Product Image WB