PRMT6 Antibody (YA685)
(Synonyms: HRMT1L6; PRMT 6)
Based on 1 Customer Validation
PRMT6 Antibody (YA685) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PRMT6.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IHC-F, IHC-P, ICC/IF
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Reactivity :
Human
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Formulation:
Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Antibody Dilution Buffer (for Western Blot)
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3-Color Prestained Protein Marker (10-190 kDa)
HY-K1011
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Ultra High Sensitivity ECL Kit
HY-K1005
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Hematoxylin-Eosin Staining Kit
HY-K0315
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High Sensitivity ECL Kit
HY-K2005
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Penicillin-Streptomycin (100×), Sterile
HY-K1006
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DPBS Buffer (1×)
HY-K3008
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GelHP Protein Gel (Tris-Gly, 10%, 15 well)
HY-K1112
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Ponceau Staining Solution (Ready-to-use, 1×)
HY-K1109
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GelHP Protein Gel (Tris-Gly, 10%, 10 well)
HY-K1111
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DAB Peroxidase Substrate Kit (Purple-Blue Color)
HY-K1107
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GelHP Protein Gel (Tris-Gly, 12%, 10 well)
HY-K1113
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
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| Dilution Ratio | 1:500-1:1000 | 1:50-1:100 | 1:50-1:100 | 1:50-1:200 |
Product Details
PRMT6 Antibody (YA685) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PRMT6.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 42 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 42 kDa
Synthetic peptide corresponding to Human PRMT6.The exact sequence is proprietary to MCE.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Hela(lane 2(20ug) , HEK293T(lane 3(20ug) and HEK293(lane 4(20ug)using PRMT6 Antibody (HY-P80875) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature. -
Immunocytochemistry analysis of Hela cells labeling PRMT6 with PRMT6 Antibody (HY-P80875) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PRMT6 Antibody (HY-P80875) at 1/100 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue). -
Immunocytochemistry analysis of Hela cells labeling PRMT6 with PRMT6 Antibody (HY-P80875) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with PRMT6 Antibody (HY-P80875) at 1/200 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Background
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Function
PRMT6 (protein arginine methyltransferase 6) is a type I protein arginine methyltransferase that catalyzes mono- and asymmetric dimethylation of arginine residues and functions predominantly as a nuclear epigenetic regulator of gene expression[1][8]. Mechanistically, PRMT6 deposits the repressive histone mark H3R2me2a, and this modification antagonizes H3K4 trimethylation, thereby influencing chromatin organization and transcriptional programs controlling cellular proliferation and differentiation[2][3]. Through regulation of H3R2 methylation at target promoters, PRMT6 modulates expression of cell-cycle regulators including p21 and p27, linking arginine methylation to G2 checkpoint control and cell-cycle progression in experimental models[4]. Beyond cell-cycle regulation, PRMT6 participates in broader epigenetic and DNA methylation networks through H3R2me2a-dependent effects on chromatin-associated regulatory machinery[8][5]. In disease contexts, dysregulated PRMT6 expression has been associated with multiple cancers, where it influences cell growth, migration, invasion, apoptosis, drug resistance, and tumor progression pathways[6][7]. Compared with related PRMT family members, PRMT6 is distinguished by its strong association with H3R2 asymmetric dimethylation and the resulting antagonistic relationship with H3K4me3-dependent transcriptional activation[2][3]. For experimental applications, increasing efforts have focused on the development of selective and non-selective PRMT6 modulators, including isoform-selective inhibitors, dual-target inhibitors, covalent inhibitors, and targeted degradation strategies, providing useful chemical tools for mechanistic studies and therapeutic target validation in cancer research[7].
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Subcellular Localization
Nucleus
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Expression
Tissue_specificity:It is highly expressed in the kidneys and testes. -
Isoforms & Post-Translational Modification
Q96LA8 has 2 isomers: Q96LA8-1: 41938 Da (predicted); Q96LA8-2: 32509 Da (predicted).
Automethylation enhances its stability and antiretroviral activity -
Subunit
Interacts with EPB41L3 and NCOA1
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SwissProt ID
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Synonyms
HRMT1L6; PRMT 6
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Research Field
Epigenetics and Nuclear Signaling
Documentation
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Data Sheet (262 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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User Guide for Antibodies (1077 KB)
[2]. Kim WY, et al. ZEITLUPE is a circadian photoreceptor stabilized by GIGANTEA in blue light. Nature. 2007 Sep 20;449(7160):356-60. [Content Brief]
[3]. Hermans A, et al. A 3D-Printed and Freely Available Device to Measure the Zebrafish Optokinetic Response Before and After Injury. Zebrafish. 2024 Apr;21(2):144-148. [Content Brief]
[4]. Kleinschmidt MA, et al. Cell cycle regulation by the PRMT6 arginine methyltransferase through repression of cyclin-dependent kinase inhibitors. PLoS One. 2012;7(8):e41446. [Content Brief]
[5]. PRMT6 gene information from NCBI.
[6]. Chen Z, et al. The Emerging Role of PRMT6 in Cancer. Front Oncol. 2022;12:841381. [Content Brief]
[7]. Rabezanahary H, et al. Live virus neutralizing antibodies against pre and post Omicron strains in food and retail workers in Québec, Canada. Heliyon. 2024 May 21;10(10):e31026. [Content Brief]
[8]. Uniprotkb.
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