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PRMT6 Antibody (YA685)

(Synonyms: HRMT1L6; PRMT 6)
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Based on 1 Customer Validation

PRMT6 Antibody (YA685) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PRMT6.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PRMT6 Antibody (YA685)
  • PRMT6 Antibody (YA685) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200

Product Details

Description

PRMT6 Antibody (YA685) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PRMT6.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database

Entrez Gene: 55170 Human

SwissProt: Q96LA8 Human

Immunogen

Synthetic peptide corresponding to Human PRMT6.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3102243

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PRMT6 Antibody (YA685)
    Western blot analysis of extracts from Hela(lane 2(20ug) , HEK293T(lane 3(20ug) and HEK293(lane 4(20ug)using PRMT6 Antibody (HY-P80875) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Arginine methyltransferase that can catalyze the formation of both omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA), with a strong preference for the formation of aDMA (PubMed:17898714, PubMed:18077460, PubMed:18079182, PubMed:19405910, PubMed:30420520). Preferentially methylates arginyl residues present in a glycine and arginine-rich domain and displays preference for monomethylated substrates (PubMed:17898714, PubMed:18077460, PubMed:18079182, PubMed:19405910). Specifically mediates the asymmetric dimethylation of histone H3 'Arg-2' to form H3R2me2a (PubMed:17898714, PubMed:18077460, PubMed:18079182). H3R2me2a represents a specific tag for epigenetic transcriptional repression and is mutually exclusive with methylation on histone H3 'Lys-4' (H3K4me2 and H3K4me3) (PubMed:17898714, PubMed:18077460). Acts as a transcriptional repressor of various genes such as HOXA2, THBS1 and TP53 (PubMed:19509293). Repression of TP53 blocks cellular senescence (By similarity). Also methylates histone H2A and H4 'Arg-3' (H2AR3me and H4R3me, respectively). Acts as a regulator of DNA base excision during DNA repair by mediating the methylation of DNA polymerase beta (POLB), leading to the stimulation of its polymerase activity by enhancing DNA binding and processivity (PubMed:16600869). Methylates HMGA1 (PubMed:16157300, PubMed:16159886). Regulates alternative splicing events. Acts as a transcriptional coactivator of a number of steroid hormone receptors including ESR1, ESR2, PGR and NR3C1. Promotes fasting-induced transcriptional activation of the gluconeogenic program through methylation of the CRTC2 transcription coactivator (By similarity). May play a role in innate immunity against HIV-1 in case of infection by methylating and impairing the function of various HIV-1 proteins such as Tat, Rev and Nucleocapsid protein p7 (NC) (PubMed:17267505). Methylates GPS2, protecting GPS2 from ubiquitination and degradation (By similarity). Methylates SIRT7, inhibiting SIRT7 histone deacetylase activity and promoting mitochondria biogenesis (PubMed:30420520)

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_specificity:It is highly expressed in the kidneys and testes.

  • Isoforms & Post-Translational Modification

    Q96LA8 has 2 isomers: Q96LA8-1: 41938 Da (predicted); Q96LA8-2: 32509 Da (predicted).
    Automethylation enhances its stability and antiretroviral activity

  • Subunit

    Interacts with EPB41L3 and NCOA1

  • SwissProt ID

    Q96LA8

  • Gene ID
    55170 [NCBI]
  • Synonyms

    HRMT1L6; PRMT 6

  • Research Field

    Epigenetics and Nuclear Signaling

PRMT6 Antibody (YA685) Related Classifications

100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    PRMT6 Antibody (YA685) purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , HEK293T(lane 3(20ug) and HEK293(lane 4(20ug)using PRMT6 Antibody (HY-P80875) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PRMT6 Antibody (YA685) Product Image
    1/16
  • Experimental Validation Results for PRMT6 Antibody (YA685)
  • PRMT6 Antibody (YA685) Product Image
  • Experimental Validation Results for PRMT6 Antibody (YA685)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) , HEK293T(lane 3(20ug) and HEK293(lane 4(20ug)using PRMT6 Antibody (HY-P80875) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PRMT6 Antibody (YA685)
  • PRMT6 Antibody (YA685) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) , HEK293T(lane 3(20ug) and HEK293(lane 4(20ug)using PRMT6 Antibody (HY-P80875) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • PRMT6 Antibody (YA685) Product Image WB