GRASP65 Antibody (YA2335)

Cat. No.: HY-P82590: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

GRASP65 Antibody (YA2335) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GRASP65.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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GRASP65 Antibody (YA2335) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

GRASP65 Antibody (YA2335) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GRASP65.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 46 kDa;

Observed band size: 65 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9BQQ3

Gene ID

64689 [NCBI]

Immunogen

A synthesized peptide derived from human GRASP65 aa400-440/440.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100
IP IP: Immunoprecipitation	1:50

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Key structural protein of the Golgi apparatus (PubMed:33301566). The membrane cisternae of the Golgi apparatus adhere to each other to form stacks, which are aligned side by side to form the Golgi ribbon (PubMed:33301566). Acting in concert with GORASP2/GRASP55, is required for the formation and maintenance of the Golgi ribbon, and may be dispensable for the formation of stacks (PubMed:33301566). However, other studies suggest that GORASP1 plays an important role in assembly and membrane stacking of the cisternae, and in the reassembly of Golgi stacks after breakdown during mitosis (By similarity). Caspase-mediated cleavage of GORASP1 is required for fragmentation of the Golgi during apoptosis (By similarity). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes (PubMed:16489344). Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane (PubMed:21884936)

Subcellular Localization：Golgi apparatus, cis-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side; Endoplasmic reticulum-Golgi intermediate compartment membrane

Isoforms & Post-Translational Modification：Q9BQQ3 has 3 isomers: Q9BQQ3-1: 46482 Da (predicted); Q9BQQ3-2: 23906 Da (predicted); Q9BQQ3-3: 26237 Da (predicted).
Phosphorylated by CDC2/B1 and PLK kinases during mitosis. Phosphorylation cycle correlates with the cisternal stacking cycle. Phosphorylation of the homodimer prevents the association of dimers into higher-order oligomers, leading to cisternal unstacking;Target for caspase-3 cleavage during apoptosis. The cleavage contributes to Golgi fragmentation and occurs very early in the execution phase of apoptosis;Myristoylated

Subunit：Homodimer. Forms higher-order oligomers under interphase but not mitotic conditions. Dimers of the protein on one membrane might be able to interact with dimers on another and so stack cisternae (PubMed:26363069). Interacts with the C-terminus of GOLGA2/GM130 under both mitotic and non-mitotic conditions (PubMed:16489344, PubMed:26363069). The interaction is critical for the correct targeting of both proteins to the cis-Golgi. Interacts with TMED2 and TMED3 (By similarity)

Database

Entrez Gene: 64689 Human

SwissProt: Q9BQQ3 Human

OMIM: 606867 Human

Research Field

Tags & Cell Markers

Synonyms

GOLPH5; GORASP1; GRASP65

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/hepg2-cell.html [Content Brief]

GRASP65 Antibody (YA2335) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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GRASP65 Antibody (YA2335)

GRASP65 Antibody (YA2335) Featured Recommendations:

Product Detail

Background

Documentation

References

GRASP65 Antibody (YA2335) Related Classifications

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