Ionotropic Glutamate Receptor 2 Antibody (YA4013)
(Synonyms: GluA2; GluR2; gluR-B; GluR-K2)Ionotropic Glutamate Receptor 2 Antibody (YA4013) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Ionotropic Glutamate Receptor 2.
-
Host:
Mouse
-
Isotype:
IgG1
-
Application:
WB, FC, ELISA
-
Reactivity :
Human
-
Formulation:
Supplied in PBS with 0.05% sodium azide
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
FC
FC: Flow Cytometry
|
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
|
|---|---|---|---|
| Dilution Ratio | 1:500-1:2000 | 1:200-1:400 | 1:10000 |
Product Details
Ionotropic Glutamate Receptor 2 Antibody (YA4013) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Ionotropic Glutamate Receptor 2.
-
Host Mouse
-
Clonality Monoclonal
-
Species ReactivityHuman
-
Observed Molecular WeightObserved band size: 99 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 99 kDa
Purified recombinant fragment of human GRIA2 (AA: 652-807) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG1
Product Properties
-
Appearance
Liquid
-
Formulation
Supplied in PBS with 0.05% sodium azide
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
Ionotropic Glutamate Receptor 2 is an Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium. The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate. Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting[1][2][3][4].
-
Subcellular Localization
Cell membrane; Multi-pass membrane protein; Postsynaptic cell membrane; Multi-pass membrane protein; Postsynaptic density membrane; Multi-pass membrane protein
-
Isoforms & Post-Translational Modification
P42262 has 4 isomers: P42262-1: 98821 Da (predicted); P42262-2: 98878 Da (predicted); P42262-3: 100594 Da (predicted); P42262-4: 93775 Da (predicted).
Palmitoylated. Depalmitoylated upon L-glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis;Ubiquitinated by RNF167, leading to its degradation;Phosphorylation at Tyr-876 is required for interaction with IQSEC1 and ARF6 activation, which in turn triggers AMPAR internalization for persistent synaptic depression;N-glycosylated -
Subunit
Homotetramer or heterotetramer of pore-forming glutamate receptor subunits (PubMed:14687553, PubMed:19651138, PubMed:20614889, PubMed:21531559, PubMed:8003671).
-
SwissProt ID
-
Synonyms
GluA2; GluR2; gluR-B; GluR-K2
Documentation
Powered by Bioz
