Phospho-Hsp27 (Ser82) Antibody

Cat. No.: HY-P80824: Data Sheet COA
SDS
User Guide for Antibodies
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Phospho-Hsp27 (Ser82) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-Hsp27 (Ser82).

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Phospho-Hsp27 (Ser82) Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Phospho-Hsp27 (Ser82) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-Hsp27 (Ser82).

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 23 kDa;

Observed band size: 27 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P04792

Gene ID

3315 [NCBI]

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser82 of Human HSP27.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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Western blot analysis of extracts from Hela(lane 2(20μg) and Hela (lane 3(20μg)), 42℃ treated (2 hours) as indicated, using Phospho-Hsp27 (Ser82) (HY-P80824) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80824, 1/1000) and Loading control antibody (Hsp27, HY-P80709, 1/1000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742)

Subcellular Localization：Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, spindle

Expression：
Tissue_specificity:The substance was detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal glands, thyroid gland, pancreas, testes, adipose tissue, kidneys, liver, spleen, cerebral cortex, serum, and cerebrospinal fluid. The highest concentrations were found in the heart and tissues composed of striated and smooth muscle.

Induction:Up-regulated in response to environmental stresses such as heat shock (PubMed:8325890) . Up-regulated by estrogen stimulation (PubMed:2743305) ; (Microbial infection) Up-regulated in response to enterovirus 71 (EV71) infection (at protein level)

Subunit：Homooligomer (PubMed:10383393). Homodimer; becomes monomeric upon activation (PubMed:20178975). Heterooligomer; with HSPB6 (PubMed:23948568). Associates with alpha- and beta-tubulin (PubMed:10777697). Interacts with TGFB1I1 (By similarity). Interacts with CRYAB (PubMed:1560006). Interacts with HSPB8 (PubMed:11342557). Interacts with HSPBAP1 (PubMed:10751411)

RRID

AB_3102212

Database

Entrez Gene: 3315 Human

SwissProt: P04792 Human

OMIM: 606595 Human

Research Field

Signal Transduction

Synonyms

HSPB1; HSP27; HSP28; Heat shock protein beta-1; HspB1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; HSP 27; Stress-responsive protein 27; SRP27

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Phospho-Hsp27 (Ser82) Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-Hsp27 (Ser82) Antibody

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