Leptin Receptor Antibody (YA2493)
(Synonyms: Leptin receptor; LEP-R; HuB219 OB receptor; OB-R; CD295; LEPR; DB; OBR)
Based on 1 Customer Validation
Leptin Receptor Antibody (YA2493) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Leptin Receptor.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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|---|---|---|---|---|
| Dilution Ratio | 1:1000-1:2000 | 1:100 | 1:500-1:1000 | 1:1000 |
Product Details
Leptin Receptor Antibody (YA2493) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Leptin Receptor.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 132 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 132 kDa
Entrez Gene: 3953 Human ; 16847 Mouse ; 24536 Rat
SwissProt: P48357 Human ; P48356 Mouse ; Q62959 Rat
OMIM: 614963 Human
Synthetic peptide within Human Leptin Receptor aa 1041-1090 / 1165.
Endogenous
Affinity Chromatography
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Leptin Receptor receptor for hormone LEP/leptin (Probable). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity. Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis. Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus. Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T -ells. Leptin increases Th1 and suppresses Th2 cytokine production; May transport LEP across the blood-brain barrier. Binds LEP and mediates LEP endocytosis. Does not induce phosphorylation of and activate STAT3; Antagonizes Isoform A and isoform B-mediated LEP binding and endocytosis[1][2][3][4][5].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein; Basolateral cell membrane; Secreted
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Expression
Tissue_specificity:Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus, but also in skeletal muscle. Detected in fundic and antral epithelial cells of the gastric mucosa (PubMed:19159218) . Isoform B and isoform A are expressed by NK cells (at protein level) (PubMed:12504075) -
Isoforms & Post-Translational Modification
P48357 has 5 isomers: P48357-1: 132494 Da (predicted); P48357-2: 102490 Da (predicted); P48357-3: 109393 Da (predicted); P48357-4: 103461 Da (predicted); P48357-5: 96042 Da (predicted).
On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation,for interaction with SOCS3 and SOCS3 mediated inhibition of leptin signaling. Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. Phosphorylation of Tyr-1079 has a more accessory role -
Subunit
Present as a mixture of monomers and dimers (Probable). The phosphorylated receptor binds a number of SH2 domain-containing proteins such as JAK2, STAT3, PTPN11, and SOCS3 (By similarity) (PubMed:9600917). Interaction with SOCS3 inhibits JAK/STAT signaling and MAPK cascade (By similarity)
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SwissProt ID
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Synonyms
Leptin receptor; LEP-R; HuB219 OB receptor; OB-R; CD295; LEPR; DB; OBR
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Research Field
Signal Transduction
Documentation
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