Copy Link Print Information

Wnt5a Antibody (YA007)

(Synonyms: WNT5A; Protein Wnt-5a)
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

Wnt5a Antibody (YA007) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Wnt5a.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Wnt5a Antibody (YA007)
  • Wnt5a Antibody (YA007) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
You May Still Need
Top Publications Citing

Use of MCE Products

Publications Citing Use of MedChemExpress (MCE) Wnt5a Antibody (YA007)

More

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:200

Product Details

Description

Wnt5a Antibody (YA007) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Wnt5a.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 45 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database

Entrez Gene: 7474 Human

SwissProt: P41221 Human

Immunogen

Synthetic peptide corresponding to Human Wnt5a aa340-354.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3662914

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Wnt5a Antibody (YA007)
    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Wnt5a Antibody (HY-P80933) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (By similarity). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed:15735754). Mediates motility of melanoma cells (PubMed:17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity)

  • Subcellular Localization

    Secreted, extracellular space, extracellular matrix; Secreted

  • Expression


    Tissue_specificity:This gene is expressed at elevated levels in differentiated thyroid carcinomas compared to normal thyroid tissue and undifferentiated thyroid tumors with low or undetectable expression levels. The gene is expressed in thyroid cells but not detected in stromal cells (protein level) (PubMed: 15735754) . It has also been detected in the hearts and lungs of newborns (PubMed: 8288227) .

  • Isoforms & Post-Translational Modification

    P41221 has 2 isomers: P41221-1: 42339 Da (predicted); P41221-2: 40887 Da (predicted).
    Glycosylation is necessary for secretion but not for activity;Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition;Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT5A

  • Subunit

    Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity (PubMed:26902720). The complex with AFM may represent the physiological form in body fluids (PubMed:26902720). Homooligomer; disulfide-linked, leading to inactivation (in vitro). Interacts with PORCN. Interacts with WLS (By similarity). Interacts with glypican GCP3 (PubMed:14610063). Interacts with PKD1 (via extracellular domain) (PubMed:27214281). Interacts with TMEM67 (PubMed:34731008)

  • SwissProt ID

    P41221

  • Gene ID
    7474 [NCBI]
  • Synonyms

    WNT5A; Protein Wnt-5a

  • Research Field

    Neuroscience

Wnt5a Antibody (YA007) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Wnt5a Antibody (HY-P80933) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Wnt5a Antibody (YA007) Product Image
    1/16
  • Experimental Validation Results for Wnt5a Antibody (YA007)
  • Wnt5a Antibody (YA007) Product Image
  • Experimental Validation Results for Wnt5a Antibody (YA007)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Wnt5a Antibody (HY-P80933) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Wnt5a Antibody (YA007)
  • Wnt5a Antibody (YA007) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Wnt5a Antibody (HY-P80933) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Wnt5a Antibody (YA007) Product Image WB