DDX1 Antibody (YA4396)(PBS only)

Cat. No.: HY-P84699A: Data Sheet User Guide for Antibodies
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DDX1 Antibody (YA4396) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DDX1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

DDX1 Antibody (YA4396) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to DDX1.

Host

Mouse

Molecular Weight

Predicted band size: 82 kDa;

Observed band size: 82 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q92499

Gene ID

1653 [NCBI]

Immunogen

Purified recombinant fragment of human DDX1 (AA: 642-740) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB: together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation (PubMed:24870230). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines via the adapter molecule TICAM1. Specifically binds (via helicase ATP-binding domain) on both short and long poly(I:C) dsRNA (By similarity); (Microbial infection) Required for HIV-1 Rev function as well as for HIV-1 and coronavirus IBV replication. Binds to the RRE sequence of HIV-1 mRNAs; (Microbial infection) Required for Coronavirus IBV replication

Subcellular Localization：Nucleus; Cytoplasm; Cytoplasmic granule; Cytoplasm, cytosol; Mitochondrion; Cytoplasm

Expression：
Tissue_specificity:The highest transcriptional levels were observed in both retinoblastoma cell lines and in neuroectodermal-derived tissues, including the retina, brain, and spinal cord.

Isoforms & Post-Translational Modification：Q92499 has 3 isomers: Q92499-1: 82432 Da (predicted); Q92499-2: 69556 Da (predicted); Q92499-3: 68712 Da (predicted).
Phosphorylated by ATM kinase; phosphorylation is increased in response to ionizing radiation (IR)

Subunit：(Microbial infection) Interacts with Venezuelan equine encephalitis virus non-structural protein 3

Synonyms

DBP-RB; UKVH5d

Documentation

User Guide for Antibodies (1077 KB)