2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. HLA-F Antibody (YA2843)

HLA-F Antibody (YA2843) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HLA-F.

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20 μL Obtener un presupuesto 4 - 5 Weeks 3 - 4 Weeks 5 - 6 Weeks 4 - 5 weeks
50 μL Obtener un presupuesto 4 - 5 Weeks 3 - 4 Weeks 5 - 6 Weeks 4 - 5 weeks
100 μL Obtener un presupuesto 4 - 5 Weeks 3 - 4 Weeks 5 - 6 Weeks 4 - 5 weeks
250 μL   Obtener un presupuesto  
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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Descripciòn

Descripciòn

HLA-F Antibody (YA2843) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HLA-F.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Peso molecular
Predicted band size: 39 kDa;
Observed band size: 42 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human HLA F aa25-76/346.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
Sensitivity Endogenous Pureza Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Envío

Shipping with blue ice.
Background
Function：Non-classical major histocompatibility class Ib molecule postulated to play a role in immune surveillance, immune tolerance and inflammation. Functions in two forms, as a heterotrimeric complex with B2M/beta-2 microglobulin and a peptide (peptide-bound HLA-F-B2M) and as an open conformer (OC) devoid of peptide and B2M (peptide-free OC). In complex with B2M, presents non-canonical self-peptides carrying post-translational modifications, particularly phosphorylated self-peptides. Peptide-bound HLA-F-B2M acts as a ligand for LILRB1 inhibitory receptor, a major player in maternal-fetal tolerance. Peptide-free OC acts as a ligand for KIR3DS1 and KIR3DL2 receptors (PubMed:28636952). Upon interaction with activating KIR3DS1 receptor on NK cells, triggers NK cell degranulation and anti-viral cytokine production (PubMed:27455421). Through interaction with KIR3DL2 receptor, inhibits NK and T cell effector functions (PubMed:24018270). May interact with other MHC class I OCs to cross-present exogenous viral, tumor or minor histompatibility antigens to cytotoxic CD8+ T cells, triggering effector and memory responses (PubMed:23851683). May play a role in inflammatory responses in the peripheral nervous system. Through interaction with KIR3DL2, may protect motor neurons from astrocyte-induced toxicity (PubMed:26928464)
Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Early endosome membrane; Lysosome membrane
Expression：
Tissue_specificity:Expression in resting B cells (protein level) . Expression in secondary lymphoid organs rich in B cells and T cells (such as tonsils, spleen, and thymus) (PubMed:10605026, PubMed:11169396) . Expression in tonsillar endothelial cells (PubMed:11169396) . Expression on activated lymphocytes (including B cells, NK cells, CD4+ T cells, and memory T cells) (PubMed:20865824, PubMed:27455421) . Expression in spinal motor neurons (PubMed:26928464) .

Induction:Up-regulated in CD4+ T cells upon stimulation via TCR and upon HIV-1 infection
Isoforms & Post-Translational Modification：P30511 has 3 isomers: P30511-1: 39062 Da (predicted); P30511-2: 28588 Da (predicted); P30511-3: 50438 Da (predicted).
N-glycosylated
Subunit：Forms a heterotrimer with B2M and a self-peptide (PubMed:28636952). Binds a diverse number of peptides ranging from 7 to more than 30 amino acids (PubMed:28636952). Peptide-bound HLA-F-B2M interacts with LILRB1 and LILRB2 but not with KIR3DS1 or KIR3DL2; this interaction is direct (PubMed:11169396, PubMed:28636952). The OC form interacts with KIR3DS1, KIR2DS4 and KIR3DL2; this interaction is direct (PubMed:24018270, PubMed:27455421, PubMed:28636952). Interacts with TAP1-TAP2 complex and CALR; this interaction is required for appropriate folding and peptide loading (PubMed:10605026, PubMed:11169396). Interacts with the coat protein complex II and 14-3-3 proteins; these interactions likely control the anterograde ER-to-Golgi transport of HLA-F (PubMed:16709803). HLA-F-B2M complex interacts with the heavy chain of other MHC class I molecules including HLA-A and HLA-E; this interaction may regulate the intracellular trafficking and the stability of peptide-free MHC class I OCs (PubMed:20483783)
Database

Entrez Gene: 3134 Human

SwissProt: P30511 Human

OMIM: 143110 Human

Research Field

Immunology
Synonyms
HLAF
Documentación
User Guide for Antibodies (1077 KB)

HLA-F Antibody (YA2843) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Nombre del producto:
HLA-F Antibody (YA2843)
Cat. No.:
HY-P83098
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