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Tropomyosin alpha 1 Chain Antibody (YA2183)

(Synonyms: TPM1; C15orf13; TMSA; Tropomyosin alpha-1 chain; Alpha-tropomyosin; Tropomyosin-1)
Customer Review

Based on 1 Customer Validation

Tropomyosin alpha 1 Chain Antibody (YA2183) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Tropomyosin alpha 1 Chain.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL IHC-P FC
  • Experimental Validation Results for Tropomyosin alpha 1 Chain Antibody (YA2183)
  • Experimental Validation Results for Tropomyosin alpha 1 Chain Antibody (YA2183)
  • Experimental Validation Results for Tropomyosin alpha 1 Chain Antibody (YA2183)
  • Tropomyosin alpha 1 Chain Antibody (YA2183) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50 1:50-1:100

Product Details

Description

Tropomyosin alpha 1 Chain Antibody (YA2183) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Tropomyosin alpha 1 Chain.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 33 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 33 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Tropomyosin 1 aa240-284/284.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104033

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Tropomyosin alpha 1 Chain Antibody (YA2183)
    Immunohistochemical analysis of paraffin-embedded rat heart tissue using Tropomyosin alpha 1 Chain Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Tropomyosin alpha 1 Chain Antibody (YA2183)
    Immunohistochemical analysis of paraffin-embedded rat heart tissue using Tropomyosin alpha 1 Chain Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Tropomyosin alpha 1 Chain Antibody (YA2183)
    Flow cytometric analysis of 1X106 Ramos cells labeling Tropomyosin alpha 1 Chain Antibody(HY-P82438, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Tropomyosin alpha 1 Chain binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments[1].

  • Subcellular Localization

    Cytoplasm, cytoskeleton

  • Expression


    Tissue_specificity:Detected in primary breast cancer tissues but undetectable in normal breast tissues in Sudanese. Isoform 1 is expressed in human skeletal muscle and cardiac tissues, with higher expression levels in the cardiac tissues. Isoform 10 is expressed in human cardiac tissues, but not in skeletal muscle

  • Isoforms & Post-Translational Modification

    P09493 has 10 isomers: P09493-1: 32709 Da (predicted); P09493-2: 26680 Da (predicted); P09493-3: 32876 Da (predicted); P09493-4: 32848 Da (predicted); P09493-5: 28385 Da (predicted); P09493-6: 32649 Da (predicted); P09493-7: 32678 Da (predicted); P09493-8: 32817 Da (predicted); P09493-9: 32737 Da (predicted); P09493-10: 32866 Da (predicted).
    Phosphorylated at Ser-283 by DAPK1 in response to oxidative stress and this phosphorylation enhances stress fiber formation in endothelial cells

  • Subunit

    Homodimer (PubMed:23170982). Heterodimer of an alpha (TPM1, TPM3 or TPM4) and a beta (TPM2) chain (By similarity).

  • SwissProt ID

    P09493

  • Gene ID
    7168 [NCBI]
  • Synonyms

    TPM1; C15orf13; TMSA; Tropomyosin alpha-1 chain; Alpha-tropomyosin; Tropomyosin-1

  • Research Field

    Signal Transduction

References

[1]. Janco M, et al. α-Tropomyosin with a D175N or E180G mutation in only one chain differs from tropomyosin with mutations in both chains. Biochemistry. 2012 Dec 11;51(49):9880-90. [Content Brief]

Tropomyosin alpha 1 Chain Antibody (YA2183) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart tissue using Tropomyosin alpha 1 Chain Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart tissue using Tropomyosin alpha 1 Chain Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Ramos cells labeling Tropomyosin alpha 1 Chain Antibody(HY-P82438, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Tropomyosin alpha 1 Chain Antibody (YA2183) Product Image
    1/16
  • Experimental Validation Results for Tropomyosin alpha 1 Chain Antibody (YA2183)
  • Experimental Validation Results for Tropomyosin alpha 1 Chain Antibody (YA2183)
  • Experimental Validation Results for Tropomyosin alpha 1 Chain Antibody (YA2183)
  • Tropomyosin alpha 1 Chain Antibody (YA2183) Product Image
  • Experimental Validation Results for Tropomyosin alpha 1 Chain Antibody (YA2183)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart tissue using Tropomyosin alpha 1 Chain Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Tropomyosin alpha 1 Chain Antibody (YA2183)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart tissue using Tropomyosin alpha 1 Chain Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Tropomyosin alpha 1 Chain Antibody (YA2183)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Ramos cells labeling Tropomyosin alpha 1 Chain Antibody(HY-P82438, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Tropomyosin alpha 1 Chain Antibody (YA2183)
  • Experimental Validation Results for Tropomyosin alpha 1 Chain Antibody (YA2183)
  • Experimental Validation Results for Tropomyosin alpha 1 Chain Antibody (YA2183)
  • Tropomyosin alpha 1 Chain Antibody (YA2183) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat heart tissue using Tropomyosin alpha 1 Chain Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Tropomyosin alpha 1 Chain Antibody (YA2183) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat heart tissue using Tropomyosin alpha 1 Chain Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Tropomyosin alpha 1 Chain Antibody (YA2183) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 Ramos cells labeling Tropomyosin alpha 1 Chain Antibody(HY-P82438, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Tropomyosin alpha 1 Chain Antibody (YA2183) Product Image IHC-P
  • Tropomyosin alpha 1 Chain Antibody (YA2183) Product Image IHC-P
  • Tropomyosin alpha 1 Chain Antibody (YA2183) Product Image FC