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COX5A Antibody (YA6513)

(Synonyms: COX antibody; COX VA antibody; COX5A antibody; COX5A_HUMAN antibody; Cytochrome c oxidase polypeptide Va antibody; Cytochrome c oxidase polypeptide, mitochondrial antibody; Cytochrome c oxidase subunit 5A antibody; Cytochrome c oxidase subunit 5A, mitochondrial antibody; Cytochrome c oxidase subunit Va antibody; mitochondrial antibody; COX antibody; COX VA antibody; COX5A antibody; COX5A_HUMAN antibody; Cytochrome c oxidase polypeptide Va antibody; Cytochrome c oxidase polypeptide, mitochondrial antibody; Cytochrome c oxidase subunit 5A antibody; Cytochrome c oxidase subunit 5A, mitochondrial antibody; Cytochrome c oxidase subunit Va antibody; mitochondrial antibody; Mitochondrial cytochrome c oxidase subunit Va antibody; VA antibody; )
Customer Review

Based on 1 Customer Validation

COX5A Antibody (YA6513) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to COX5A.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for COX5A Antibody (YA6513)
  • COX5A Antibody (YA6513) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:5000 1:1000

Product Details

Description

COX5A Antibody (YA6513) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to COX5A.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 13 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 17 kDa
Immunogen

Recombinant protein within human COX5A aa 1-150.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719218

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for COX5A Antibody (YA6513)
    Western blot analysis of extracts from Hela (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using COX5A Antibody (HY-P86820) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    COX5A component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix

  • Subcellular Localization

    Mitochondrion inner membrane

  • Isoforms & Post-Translational Modification

    P20674: 150 amino acids, molecular weight 16762 Da.
    Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression丨Acetylated on Lys-5, Lys-8, Lys-12 and Lys-14 by KAT2A; KAT2A is recruited by the XPC complex in absence of DNA damage (PubMed:31527837)丨Monomethylated on Lys-5 and Lys-8 by SETD6丨Not phosphorylated丨Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription

  • Subunit

    Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of 14 subunits

  • SwissProt ID

    P20674

  • Gene ID
    9377 [NCBI]
  • Synonyms

    COX antibody; COX VA antibody; COX5A antibody; COX5A_HUMAN antibody; Cytochrome c oxidase polypeptide Va antibody; Cytochrome c oxidase polypeptide, mitochondrial antibody; Cytochrome c oxidase subunit 5A antibody; Cytochrome c oxidase subunit 5A, mitochondrial antibody; Cytochrome c oxidase subunit Va antibody; mitochondrial antibody; COX antibody; COX VA antibody; COX5A antibody; COX5A_HUMAN antibody; Cytochrome c oxidase polypeptide Va antibody; Cytochrome c oxidase polypeptide, mitochondrial antibody; Cytochrome c oxidase subunit 5A antibody; Cytochrome c oxidase subunit 5A, mitochondrial antibody; Cytochrome c oxidase subunit Va antibody; mitochondrial antibody; Mitochondrial cytochrome c oxidase subunit Va antibody; VA antibody;

COX5A Antibody (YA6513) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using COX5A Antibody (HY-P86820) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • COX5A Antibody (YA6513) Product Image
    1/16
  • Experimental Validation Results for COX5A Antibody (YA6513)
  • COX5A Antibody (YA6513) Product Image
  • Experimental Validation Results for COX5A Antibody (YA6513)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using COX5A Antibody (HY-P86820) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for COX5A Antibody (YA6513)
  • COX5A Antibody (YA6513) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using COX5A Antibody (HY-P86820) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • COX5A Antibody (YA6513) Product Image WB