COX5A Antibody (YA6513)

Cat. No.: HY-P86820: Data Sheet COA
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COX5A Antibody (YA6513) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to COX5A.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

COX5A Antibody (YA6513) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to COX5A.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 17 kDa;

Observed band size: 13 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P20674

Gene ID

9377 [NCBI]

Immunogen

Recombinant protein within human COX5A aa 1-150.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:5000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:1000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、Mouse brain (lane 3(20μg)) and Rat brain (lane 4(20μg)) using COX5A Antibody (HY-P86820) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Tubulin, 1/5000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

Function：Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix

Subcellular Localization：Mitochondrion inner membrane

Isoforms & Post-Translational Modification：P20674: 150 amino acids, molecular weight 16762 Da.
Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression丨Acetylated on Lys-5, Lys-8, Lys-12 and Lys-14 by KAT2A; KAT2A is recruited by the XPC complex in absence of DNA damage (PubMed:31527837)丨Monomethylated on Lys-5 and Lys-8 by SETD6丨Not phosphorylated丨Lactylated in macrophages by EP300/P300 by using lactoyl-CoA directly derived from endogenous or exogenous lactate, leading to stimulates gene transcription

Subunit：Component of the cytochrome c oxidase (complex IV, CIV), a multisubunit enzyme composed of 14 subunits

RRID

AB_3719218

Synonyms

COX antibody; COX VA antibody; COX5A antibody; COX5A_HUMAN antibody; Cytochrome c oxidase polypeptide Va antibody; Cytochrome c oxidase polypeptide, mitochondrial antibody; Cytochrome c oxidase subunit 5A antibody; Cytochrome c oxidase subunit 5A, mitochondrial antibody; Cytochrome c oxidase subunit Va antibody; mitochondrial antibody; COX antibody; COX VA antibody; COX5A antibody; COX5A_HUMAN antibody; Cytochrome c oxidase polypeptide Va antibody; Cytochrome c oxidase polypeptide, mitochondrial antibody; Cytochrome c oxidase subunit 5A antibody; Cytochrome c oxidase subunit 5A, mitochondrial antibody; Cytochrome c oxidase subunit Va antibody; mitochondrial antibody; Mitochondrial cytochrome c oxidase subunit Va antibody; VA antibody;

Documentation

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Data Sheet (231 KB) SDS (253 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)