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Cat. No.: HY-P87863

Thioredoxin Antibody (YA7548)

Thioredoxin Antibody (YA7548) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Thioredoxin.

For research use only. We do not sell to patients.

Formulation:
Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

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Amount: USD 0.00

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications

Application	IHC-P IHC-P: Immunohistochemistry-Paraffin	WB WB: Western Blot	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence	ELISA ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio	1:500-1:2000	1:2000-1:10000	1:200-1:1000	1:5000-1:20000

Description

Thioredoxin Antibody (YA7548) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Thioredoxin.

Host Rabbit
Clonality Monoclonal,Recombinant
Species Reactivity
Human, Mouse, Rat
Observed Molecular Weight

Observed band size: 12 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 12 kDa

Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG,Kappa

Appearance

Liquid
Formulation

Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Function

Thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity |ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55)^[1]^[2]^[3]^[4]^[5]^[6]^[7].
Subcellular Localization

Nucleus,Cytoplasm,Secreted
Expression

Induction: Up-regulated by ionizing radiation
Isoforms & Post-Translational Modification

P10599 has two isomers: P10599-1: 11737 Da (predicted); P10599-2: 9452 Da (predicted).
In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO)丨In case of infection, ubiquitinated by S
Subunit

Homodimer; disulfide-linked (PubMed:17260951, PubMed:9369469)
SwissProt ID

P10599