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Myosin Light Chain 2 Antibody (YA2709)

(Synonyms: Myosin regulatory light chain 2; ventricular/cardiac muscle isoform; MLC-2; MLC-2v)
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Based on 1 Customer Validation

Myosin Light Chain 2 Antibody (YA2709) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myosin Light Chain 2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Myosin Light Chain 2 Antibody (YA2709)
  • Experimental Validation Results for Myosin Light Chain 2 Antibody (YA2709)
  • Myosin Light Chain 2 Antibody (YA2709) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50

Product Details

Description

Myosin Light Chain 2 Antibody (YA2709) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Myosin Light Chain 2.

  • Host Rabbit
  • Clonality Recombinant, Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 19 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 19 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human Myosin Light Chain 2 aa100-166/166.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105083

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Myosin Light Chain 2 Antibody (YA2709)
    Immunohistochemical analysis of paraffin-embedded rat heart tissue using MYL2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myosin Light Chain 2 Antibody (YA2709)
    Immunohistochemical analysis of paraffin-embedded rat heart tissue using MYL2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Myosin Light Chain 2 is a Contractile protein that plays a role in heart development and function. Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force. During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly[1][2].

  • Subcellular Localization

    Cytoplasm, myofibril, sarcomere, A band

  • Expression


    Tissue_specificity:High expression in type I myofibrils

  • Subunit

    Myosin is a hexamer of 2 heavy chains and 4 light chains. Interacts with MYOC (PubMed:11773029)

  • SwissProt ID

    P10916

  • Gene ID
    4633 [NCBI]
  • Synonyms

    Myosin regulatory light chain 2; ventricular/cardiac muscle isoform; MLC-2; MLC-2v

  • Research Field

    Signal Transduction

Myosin Light Chain 2 Antibody (YA2709) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart tissue using MYL2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart tissue using MYL2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myosin Light Chain 2 Antibody (YA2709) Product Image
    1/16
  • Experimental Validation Results for Myosin Light Chain 2 Antibody (YA2709)
  • Experimental Validation Results for Myosin Light Chain 2 Antibody (YA2709)
  • Myosin Light Chain 2 Antibody (YA2709) Product Image
  • Experimental Validation Results for Myosin Light Chain 2 Antibody (YA2709)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart tissue using MYL2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myosin Light Chain 2 Antibody (YA2709)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded rat heart tissue using MYL2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Myosin Light Chain 2 Antibody (YA2709)
  • Experimental Validation Results for Myosin Light Chain 2 Antibody (YA2709)
  • Myosin Light Chain 2 Antibody (YA2709) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat heart tissue using MYL2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myosin Light Chain 2 Antibody (YA2709) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded rat heart tissue using MYL2 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Myosin Light Chain 2 Antibody (YA2709) Product Image IHC-P
  • Myosin Light Chain 2 Antibody (YA2709) Product Image IHC-P