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PD-L2 Antibody (YA3391)

(Synonyms: PD-1 ligand 2; PD-L2; PDCD1 ligand 2; B7-DC; CD273; PD L2; PDL2)
Customer Review

Based on 1 Customer Validation

PD-L2 Antibody (YA3391) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to PD-L2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2b

  • Application:

    WB, ICC/IF, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for PD-L2 Antibody (YA3391)
  • PD-L2 Antibody (YA3391) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:1000 1:50-1:200 1:50-1:100 1:10000

Product Details

Description

PD-L2 Antibody (YA3391) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to PD-L2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 35-70kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 31 kDa
Species Reactivity Database
Immunogen

Purified recombinant fragment of human CD273 aa 20-220.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2b

RRID

AB_3105042

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PD-L2 Antibody (YA3391)
    Western blot analysis was performed on extracts from U2OS (lane 1, 20 μg), 3T3 (lane 2, 20 μg), and Raji (lane 3, 20 μg) using PDL2 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin,HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

  • Function

    PD-L2 is involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production

  • Subcellular Localization

    Secreted; Endomembrane system; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:It is highly expressed in the heart, placenta, pancreas, lungs, and liver, and lowly expressed in the spleen, lymph nodes, and thymus.

    Induction:Up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL4/interleukin-4

  • Isoforms & Post-Translational Modification

    Q9BQ51 has 3 isomers: Q9BQ51-1: 30957 Da (predicted); Q9BQ51-2: 20867 Da (predicted); Q9BQ51-3: 20745 Da (predicted).

  • Subunit

    Interacts with PDCD1

  • SwissProt ID

    Q9BQ51

  • Gene ID
    80380 [NCBI]
  • Synonyms

    PD-1 ligand 2; PD-L2; PDCD1 ligand 2; B7-DC; CD273; PD L2; PDL2

  • Research Field

    Immunology

PD-L2 Antibody (YA3391) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from U2OS (lane 1, 20 μg), 3T3 (lane 2, 20 μg), and Raji (lane 3, 20 μg) using PDL2 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin,HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PD-L2 Antibody (YA3391) Product Image
    1/16
  • Experimental Validation Results for PD-L2 Antibody (YA3391)
  • PD-L2 Antibody (YA3391) Product Image
  • Experimental Validation Results for PD-L2 Antibody (YA3391)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from U2OS (lane 1, 20 μg), 3T3 (lane 2, 20 μg), and Raji (lane 3, 20 μg) using PDL2 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin,HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for PD-L2 Antibody (YA3391)
  • PD-L2 Antibody (YA3391) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from U2OS (lane 1, 20 μg), 3T3 (lane 2, 20 μg), and Raji (lane 3, 20 μg) using PDL2 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin,HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • PD-L2 Antibody (YA3391) Product Image WB