ANG Antibody
(Synonyms: RNASE5, ANG, Angiogenin, Ribonuclease 5, RNase 5)ANG Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ANG.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
ANG Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ANG.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 16 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 16 kDa
Synthetic peptide corresponding to the center region of human ANG.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ANG secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context. Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus. Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs): specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation. tiRNas also prevent formation of apoptosome, thereby promoting cell survival (By similarity). Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36. Cleaves a subset of tRNAs, including tRNA(Ala), tRNA(Glu), tRNA(Gly), tRNA(Lys), tRNA(Val), tRNA(His), tRNA(Asp) and tRNA(Sec). Under growth conditions and in differentiated cells, translocates to the nucleus and stimulates ribosomal RNA (rRNA) transcription, including that containing the initiation site sequences of 45S rRNA, thereby promoting cell growth and proliferation. Angiogenin induces vascularization of normal and malignant tissues via its ability to promote rRNA transcription. Involved in hematopoietic stem and progenitor cell (HSPC) growth and survival by promoting rRNA transcription in growth conditions and inhibiting translation in response to stress, respectively. Mediates the crosstalk between myeloid and intestinal epithelial cells to protect the intestinal epithelial barrier integrity: secreted by myeloid cells and promotes intestinal epithelial cells proliferation and survival. Also mediates osteoclast-endothelial cell crosstalk in growing bone: produced by osteoclasts and protects the neighboring vascular cells against senescence by promoting rRNA transcription (By similarity)[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28].
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Subcellular Localization
Secreted; Nucleus; Nucleus, nucleolus; Cytoplasm, Stress granule
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Expression
Tissue_Specificity: Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. -
Isoforms & Post-Translational Modification
ANG has an amino acid length of 147, molecular weight is 16550 Da.
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Subunit
Homodimer.
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SwissProt ID
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Synonyms
RNASE5, ANG, Angiogenin, Ribonuclease 5, RNase 5
Documentation
[1]. Xu ZP, et al. The nuclear function of angiogenin in endothelial cells is related to rRNA production. Biochem Biophys Res Commun. 2002 Jun 7;294(2):287-92. [Content Brief]
[2]. Saxena SK, et al. Angiogenin is a cytotoxic, tRNA-specific ribonuclease in the RNase A superfamily. J Biol Chem. 1992 Oct 25;267(30):21982-6. [Content Brief]
[3]. Yamasaki S, et al. Angiogenin cleaves tRNA and promotes stress-induced translational repression. J Cell Biol. 2009 Apr 6;185(1):35-42. [Content Brief]
[4]. Emara MM, et al. Angiogenin-induced tRNA-derived stress-induced RNAs promote stress-induced stress granule assembly. J Biol Chem. 2010 Apr 2;285(14):10959-68. [Content Brief]
[5]. Ivanov P, et al. Angiogenin-induced tRNA fragments inhibit translation initiation. Mol Cell. 2011 Aug 19;43(4):613-23. [Content Brief]
[6]. Thiyagarajan N, et al. Structural and molecular insights into the mechanism of action of human angiogenin-ALS variants in neurons. Nat Commun. 2012;3:1121. [Content Brief]
[7]. Pizzo E, et al. Ribonuclease/angiogenin inhibitor 1 regulates stress-induced subcellular localization of angiogenin to control growth and survival. J Cell Sci. 2013 Sep 15;126(Pt 18):4308-19. [Content Brief]
[8]. Shapiro R, et al. Characteristic ribonucleolytic activity of human angiogenin. Biochemistry. 1986 Jun 17;25(12):3527-32. [Content Brief]
[9]. Harper JW, et al. Mutagenesis of aspartic acid-116 enhances the ribonucleolytic activity and angiogenic potency of angiogenin. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7139-43. [Content Brief]
[10]. Lee FS, et al. Characterization of ribonucleolytic activity of angiogenin towards tRNA. Biochem Biophys Res Commun. 1989 May 30;161(1):121-6. [Content Brief]
[11]. Goncalves KA, et al. Angiogenin Promotes Hematopoietic Regeneration by Dichotomously Regulating Quiescence of Stem and Progenitor Cells. Cell. 2016 Aug 11;166(4):894-906. [Content Brief]
[12]. Bradshaw WJ, et al. Structural insights into human angiogenin variants implicated in Parkinson's disease and Amyotrophic Lateral Sclerosis. Sci Rep. 2017 Feb 8;7:41996. [Content Brief]
[13]. Yu W, et al. Plexin-B2 Mediates Physiologic and Pathologic Functions of Angiogenin. Cell. 2017 Nov 2;171(4):849-864.e25. [Content Brief]
[14]. Thomas SP, et al. Human angiogenin is a potent cytotoxin in the absence of ribonuclease inhibitor. RNA. 2018 Aug;24(8):1018-1027. [Content Brief]
[15]. Shapiro R, et al. Ribonucleolytic activity of angiogenin: essential histidine, lysine, and arginine residues. Proc Natl Acad Sci U S A. 1987 Dec;84(24):8783-7. [Content Brief]
[16]. Bai R, et al. Myeloid cells protect intestinal epithelial barrier integrity through the angiogenin/plexin-B2 axis. EMBO J. 2020 Jul 1;39(13):e103325. [Content Brief]
[17]. Loveland AB, et al. Structural mechanism of angiogenin activation by the ribosome. Nature. 2024 Jun;630(8017):769-776. [Content Brief]
[18]. Russo N, et al. Role of glutamine-117 in the ribonucleolytic activity of human angiogenin. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):2920-4. [Content Brief]
[19]. Russo N, et al. A combined kinetic and modeling study of the catalytic center subsites of human angiogenin. Proc Natl Acad Sci U S A. 1996 Jan 23;93(2):804-8. [Content Brief]
[20]. Russo N, et al. The C-terminal region of human angiogenin has a dual role in enzymatic activity. Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3243-7. [Content Brief]
[21]. Shapiro R, et al. Structural features that determine the enzymatic potency and specificity of human angiogenin: threonine-80 and residues 58-70 and 116-123. Biochemistry. 1998 May 12;37(19):6847-56. [Content Brief]
[22]. Rybak SM, et al. Base cleavage specificity of angiogenin with Saccharomyces cerevisiae and Escherichia coli 5S RNAs. Biochemistry. 1988 Apr 5;27(7):2288-94. [Content Brief]
[23]. Su Z, et al. Angiogenin generates specific stress-induced tRNA halves and is not involved in tRF-3-mediated gene silencing. J Biol Chem. 2019 Nov 8;294(45):16930-16941. [Content Brief]
[24]. Tsuji T, et al. Angiogenin is translocated to the nucleus of HeLa cells and is involved in ribosomal RNA transcription and cell proliferation. Cancer Res. 2005 Feb 15;65(4):1352-60. [Content Brief]
[25]. Moroianu J, et al. Nuclear translocation of angiogenin in proliferating endothelial cells is essential to its angiogenic activity. Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1677-81. [Content Brief]
[26]. Dickson KA, et al. Ribonuclease inhibitor regulates neovascularization by human angiogenin. Biochemistry. 2009 May 12;48(18):3804-6. [Content Brief]
[27]. Fett JW, et al. Isolation and characterization of angiogenin, an angiogenic protein from human carcinoma cells. Biochemistry. 1985 Sep 24;24(20):5480-6. [Content Brief]
[28]. Bond MD, et al. Characterization and sequencing of rabbit, pig and mouse angiogenins: discernment of functionally important residues and regions. Biochim Biophys Acta. 1993 Mar 5;1162(1-2):177-86. [Content Brief]
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