Characterization of ribonucleolytic activity of angiogenin towards tRNA

  • Biochem Biophys Res Commun. 1989 May 30;161(1):121-6. doi: 10.1016/0006-291x(89)91569-6.
F S Lee  1 ,  B L Vallee
Affiliations
  • 1. Center for Biochemical and Biophysical Sciences and Medicine, Harvard Medical School, Boston, Massachusetts 02115.
PMID: 2730651 DOI: 10.1016/0006-291x(89)91569-6
Abstract

Yeast tRNA is a convenient substrate for the assay of the ribonucleolytic activity of human angiogenin. The optimal pH, [NaCl], and temperature for tRNA cleavage by angiogenin are approximately 6.8, 15-30 mM, and approximately 55 degrees C, respectively, as compared with approximately 8.0, 100-200 mM, and approximately 65 degrees C, respectively, for RNase A. Polyanions and metals both inhibit angiogenin and RNase A but to different extents.