USP30 Antibody
(Synonyms: Ubiquitin carboxyl-terminal hydrolase 30, Deubiquitinating enzyme 30, Ubiquitin thioesterase 30, Ubiquitin-specific-processing protease 30, Ub-specific protease 30, USP30)USP30 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to USP30.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, ICC/IF
-
Reactivity :
Human, Mouse, Rat
-
Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-500 |
Product Details
USP30 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to USP30.
-
Host Rabbit
-
Clonality Polyclonal
-
Species ReactivityHuman, Mouse, Rat
-
Observed Molecular WeightObserved band size: 59 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 58 kDa
Synthetic peptide corresponding to the N-term region of human USP30.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Livraison
Shipping with blue ice.
Background
-
Function
USP30 is a Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy. Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling. Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65'. Acts as a negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2 (By similarity)[1][2][3][4].
-
Subcellular Localization
Mitochondrion outer membrane
-
Expression
Tissue_Specificity: Expressed in skeletal muscle, pancreas, liver and kidney. -
Isoforms & Post-Translational Modification
USP30 has an amino acid length of 517, molecular weight is 58503 Da.第 235 位赖氨酸(Lys-235)和第 289 位赖氨酸(Lys-289)可被帕金蛋白(PRKN)泛素化，进而导致其降解USP30 的氨基酸个数为 517 个，分子量为 58503 Da。Ubiquitinated by parkin (PRKN) at Lys-235 and Lys-289, leading to its degradation
-
SwissProt ID
-
Synonyms
Ubiquitin carboxyl-terminal hydrolase 30, Deubiquitinating enzyme 30, Ubiquitin thioesterase 30, Ubiquitin-specific-processing protease 30, Ub-specific protease 30, USP30
Documentation
[1]. Nakamura N, et al. Regulation of mitochondrial morphology by USP30, a deubiquitinating enzyme present in the mitochondrial outer membrane. Mol Biol Cell. 2008 May;19(5):1903-11. [Content Brief]
[2]. Bingol B, et al. The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy. Nature. 2014 Jun 19;510(7505):370-5. [Content Brief]
[3]. Wauer T, et al. Ubiquitin Ser65 phosphorylation affects ubiquitin structure, chain assembly and hydrolysis. EMBO J. 2015 Feb 3;34(3):307-25. [Content Brief]
[4]. Cunningham CN, et al. USP30 and parkin homeostatically regulate atypical ubiquitin chains on mitochondria. Nat Cell Biol. 2015 Feb;17(2):160-9. [Content Brief]
Powered by Bioz
