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CD31 Antibody (YA806)

(Synonyms: Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31)
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Based on 1 publication(s) in Google Scholar

CD31 Antibody (YA806) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CD31.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2a

  • Application:

    WB, ICC/IF, IHC-P, mIHC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD31 Antibody (YA806)
  • Experimental Validation Results for CD31 Antibody (YA806)
  • CD31 Antibody (YA806) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
mIHC Info
mIHC: Multiplex Immunohistochemical
Dilution Ratio 1:1000-1:5000 1:50-1:200 1:500-1:2000 1:1000-1:3000

Product Details

Description

CD31 Antibody (YA806) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CD31.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 130 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 83 kDa
Species Reactivity Database
Immunogen

Synthetic peptide (KLH-coupled) within C-terminal residues of human CD31.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2a

RRID

AB_3102929

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD31 Antibody (YA806)
    Western blot analysis of extracts from THP-1 (lane 2(20μg) and THP-1 (lane 3(40μg)using CD31 (HY-P80599) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80599, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD31 Antibody (YA806)
    Immunocytochemistry analysis of NIH3T3 cells labeling CD31 with CD31 antibody (HY-P80599) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD31 antibody (HY-P80599) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:17580308, PubMed:19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity); Does not protect against apoptosis

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Membrane raft; Cell junction; Cell junction

  • Expression


    Tissue_specificity:This protein is expressed on platelets and leukocytes, primarily concentrated at the boundaries between endothelial cells (PubMed:18388311, PubMed:21464369) . It is also expressed (protein level) in human umbilical vein endothelial cells (HUVECs) (PubMed:17580308, PubMed:19342684) . It is also expressed (protein level) on neutrophils (PubMed:17580308) . The long subtype predominated in all tissues examined (PubMed:12433657) . The Δ12 subtype was detected only in the trachea (PubMed:12433657) . The Δ14-15 subtype was detected only in the lungs (PubMed:12433657) . The Delta14 subtype was detected in all tissues examined, with the strongest expression in the heart (PubMed:12433657) . Delta15 is expressed (protein level) in the brain, testes, ovaries, platelet cells, human umbilical vein endothelial cells (HUVECs) , Jurkat T-cell leukemia, human erythroleukemia (HEL) , and U-937 histiocytic lymphoma cell lines (PubMed:12433657, PubMed:18388311) .

  • Isoforms & Post-Translational Modification

    P16284 has 6 isomers: P16284-1: 82522 Da (predicted); P16284-2: 80465 Da (predicted); P16284-3: 80193 Da (predicted); P16284-4: 80405 Da (predicted); P16284-5: 79247 Da (predicted); P16284-6: 81364 Da (predicted).
    Phosphorylated on Ser and Tyr residues after cellular activation by src kinases (PubMed:18710921, PubMed:19342684, PubMed:21464369, PubMed:9298995). Upon activation, phosphorylated on Ser-729 which probably initiates the dissociation of the membrane-interaction segment (residues 709-729) from the cell membrane allowing the sequential phosphorylation of Tyr-713 and Tyr-690 (PubMed:21464369). Constitutively phosphorylated on Ser-734 in resting platelets (PubMed:21464369). Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation (PubMed:18710921);Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts

  • Subunit

    Trans-homodimer (via Ig-like C2-type 1 and Ig-like C2-type 2 domains); trans-homodimerization is required for cell-cell interaction (PubMed:26702061, PubMed:27958302). Forms a complex with BDKRB2 and GNAQ (PubMed:18672896). Interacts with BDKRB2 and GNAQ (PubMed:18672896). Interacts with PTPN11; Tyr-713 is critical for PTPN11 recruitment (PubMed:18388311, PubMed:19342684). Interacts with FER (By similarity). Interacts (via Ig-like C2-type domain 6) with CD177; the interaction is Ca(2+)-dependent; the interaction is direct (PubMed:17580308)

  • SwissProt ID

    P16284

  • Gene ID
    5175 [NCBI]
  • Synonyms

    Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31

  • Research Field

    Cardiovascular

CD31 Antibody (YA806) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from THP-1 (lane 2(20μg) and THP-1 (lane 3(40μg)using CD31 (HY-P80599) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80599, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of NIH3T3 cells labeling CD31 with CD31 antibody (HY-P80599) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD31 antibody (HY-P80599) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CD31 Antibody (YA806) Product Image
    1/16
  • Experimental Validation Results for CD31 Antibody (YA806)
  • Experimental Validation Results for CD31 Antibody (YA806)
  • CD31 Antibody (YA806) Product Image
  • Experimental Validation Results for CD31 Antibody (YA806)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from THP-1 (lane 2(20μg) and THP-1 (lane 3(40μg)using CD31 (HY-P80599) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80599, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD31 Antibody (YA806)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of NIH3T3 cells labeling CD31 with CD31 antibody (HY-P80599) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD31 antibody (HY-P80599) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for CD31 Antibody (YA806)
  • Experimental Validation Results for CD31 Antibody (YA806)
  • CD31 Antibody (YA806) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from THP-1 (lane 2(20μg) and THP-1 (lane 3(40μg)using CD31 (HY-P80599) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80599, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CD31 Antibody (YA806) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of NIH3T3 cells labeling CD31 with CD31 antibody (HY-P80599) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD31 antibody (HY-P80599) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • CD31 Antibody (YA806) Product Image WB
  • CD31 Antibody (YA806) Product Image ICC