CD31 Antibody (YA806)

Cat. No.: HY-P80599: Data Sheet COA
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CD31 Antibody (YA806) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CD31.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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CD31 Antibody (YA806) Featured Recommendations:

1 Publications Citing Use of MCE CD31 Antibody (YA806)

•Mol Med. 2025 Nov 18;31(1):332. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

CD31 Antibody (YA806) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to CD31.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 83 kDa;

Observed band size: 130 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P16284

Gene ID

5175 [NCBI]

Immunogen

Synthetic peptide (KLH-coupled) within C-terminal residues of human CD31.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-1:5000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:500-1:2000
mIHC mIHC: Multiplex Immunohistochemical	1:1000-1:3000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB ICC

Western blot analysis of extracts from THP-1 (lane 2(20μg) and THP-1 (lane 3(40μg)using CD31 (HY-P80599) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% defatted milk powder in TBST for 2 hour at room temperature. The primary antibody (HY-P80599, 1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% defatted milk powder in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Immunocytochemistry analysis of NIH3T3 cells labeling CD31 with CD31 antibody (HY-P80599) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with CD31 antibody (HY-P80599) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:17580308, PubMed:19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity); Does not protect against apoptosis

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Membrane raft; Cell junction; Cell junction

Expression：
Tissue_specificity:This protein is expressed on platelets and leukocytes, primarily concentrated at the boundaries between endothelial cells (PubMed:18388311, PubMed:21464369) . It is also expressed (protein level) in human umbilical vein endothelial cells (HUVECs) (PubMed:17580308, PubMed:19342684) . It is also expressed (protein level) on neutrophils (PubMed:17580308) . The long subtype predominated in all tissues examined (PubMed:12433657) . The Δ12 subtype was detected only in the trachea (PubMed:12433657) . The Δ14-15 subtype was detected only in the lungs (PubMed:12433657) . The Delta14 subtype was detected in all tissues examined, with the strongest expression in the heart (PubMed:12433657) . Delta15 is expressed (protein level) in the brain, testes, ovaries, platelet cells, human umbilical vein endothelial cells (HUVECs) , Jurkat T-cell leukemia, human erythroleukemia (HEL) , and U-937 histiocytic lymphoma cell lines (PubMed:12433657, PubMed:18388311) .

Isoforms & Post-Translational Modification：P16284 has 6 isomers: P16284-1: 82522 Da (predicted); P16284-2: 80465 Da (predicted); P16284-3: 80193 Da (predicted); P16284-4: 80405 Da (predicted); P16284-5: 79247 Da (predicted); P16284-6: 81364 Da (predicted).
Phosphorylated on Ser and Tyr residues after cellular activation by src kinases (PubMed:18710921, PubMed:19342684, PubMed:21464369, PubMed:9298995). Upon activation, phosphorylated on Ser-729 which probably initiates the dissociation of the membrane-interaction segment (residues 709-729) from the cell membrane allowing the sequential phosphorylation of Tyr-713 and Tyr-690 (PubMed:21464369). Constitutively phosphorylated on Ser-734 in resting platelets (PubMed:21464369). Phosphorylated on tyrosine residues by FER and FES in response to FCER1 activation (By similarity). In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation (PubMed:18710921);Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts

Subunit：Trans-homodimer (via Ig-like C2-type 1 and Ig-like C2-type 2 domains); trans-homodimerization is required for cell-cell interaction (PubMed:26702061, PubMed:27958302). Forms a complex with BDKRB2 and GNAQ (PubMed:18672896). Interacts with BDKRB2 and GNAQ (PubMed:18672896). Interacts with PTPN11; Tyr-713 is critical for PTPN11 recruitment (PubMed:18388311, PubMed:19342684). Interacts with FER (By similarity). Interacts (via Ig-like C2-type domain 6) with CD177; the interaction is Ca(2+)-dependent; the interaction is direct (PubMed:17580308)

RRID

AB_3102929

Database

Entrez Gene: 5175 Human ; 18613 Mouse ; 29583 Rat

SwissProt: P16284 Human ; Q08481 Mouse ; Q3SWT0 Rat

OMIM: 173445 Human

Research Field

Cardiovascular

Synonyms

Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

CD31 Antibody (YA806) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CD31 Antibody (YA806)

CD31 Antibody (YA806) Featured Recommendations:

1 Publications Citing Use of MCE CD31 Antibody (YA806)

Product Detail

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Background

Documentation

CD31 Antibody (YA806) Related Classifications

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