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RANK Antibody (YA4604)

(Synonyms: FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1; TRANCE-R)
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Based on 1 Customer Validation

RANK Antibody (YA4604) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RANK.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    FC, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for RANK Antibody (YA4604)
  • RANK Antibody (YA4604) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:400 1:10000

Product Details

Description

RANK Antibody (YA4604) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RANK.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 80 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 66 kDa
Immunogen

Purified recombinant fragment of human TNFRSF11A (AA: extra 30-212) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RANK Antibody (YA4604)
    Flow cytometric analysis of 1X10^6 HL-60 cells labeling TNFRSF11A Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

Background

  • Function

    RANK receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Its interaction with EEIG1 promotes osteoclastogenesis via facilitating the transcription of NFATC1 and activation of PLCG2. Involved in the regulation of interactions between T-cells and dendritic cells[1].

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Membrane raft; Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:This gene is widely expressed in various tissues throughout the body, with high expression levels in skeletal muscle, thymus, liver, colon, small intestine, and adrenal glands.

  • Isoforms & Post-Translational Modification

    Q9Y6Q6 has 6 isomers: Q9Y6Q6-1: 66034 Da (predicted); Q9Y6Q6-2: 32318 Da (predicted); Q9Y6Q6-3: 36399 Da (predicted); Q9Y6Q6-4: 28965 Da (predicted); Q9Y6Q6-5: 56402 Da (predicted); Q9Y6Q6-6: 64552 Da (predicted).

  • Subunit

    Binds to the clefts between the subunits of the TNFSF11 ligand trimer to form a heterohexamer (By similarity). Part of a complex composed of EEIG1, TNFRSF11A/RANK, PLCG2, GAB2, TEC and BTK; complex formation increases in the presence of TNFSF11/RANKL (PubMed:23478294). Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6 (PubMed:9774460). Interacts (via cytoplasmic domain) with GAB2 (PubMed:15750601). Interacts (via cytoplasmic domain); with EEIG1 (via N-terminus); when in the presence of TNFSF11/RANKL (By similarity)

  • SwissProt ID

    Q9Y6Q6

  • Gene ID
    8792 [NCBI]
  • Synonyms

    FEO; OFE; ODFR; OSTS; PDB2; RANK; CD265; OPTB7; TRANCER; LOH18CR1; TRANCE-R

RANK Antibody (YA4604) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HL-60 cells labeling TNFRSF11A Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • RANK Antibody (YA4604) Product Image
    1/16
  • Experimental Validation Results for RANK Antibody (YA4604)
  • RANK Antibody (YA4604) Product Image
  • Experimental Validation Results for RANK Antibody (YA4604)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HL-60 cells labeling TNFRSF11A Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).
  • Experimental Validation Results for RANK Antibody (YA4604)
  • RANK Antibody (YA4604) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 HL-60 cells labeling TNFRSF11A Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • RANK Antibody (YA4604) Product Image FC