CD127 Antibody (YA545)

Cat. No.: HY-P80593: Data Sheet COA
SDS
User Guide for Antibodies
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CD127 Antibody (YA545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD127.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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CD127 Antibody (YA545) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

CD127 Antibody (YA545) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD127.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 52 kDa;

Observed band size: 70 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P16871

Gene ID

3575 [NCBI]

Immunogen

Synthetic peptide corresponding to Human CD127.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Daudi (lane 1, 20 μg), HeLa (lane 2, 20 μg), U2OS (lane 3, 20 μg), and K562 (lane 4, 20 μg) using CD127 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein; Cell membrane; Single-pass type I membrane protein; Secreted

Isoforms & Post-Translational Modification：P16871 has 4 isomers: P16871-1: 51579 Da (predicted); P16871-2: 34018 Da (predicted); P16871-3: 29936 Da (predicted); P16871-4: 28722 Da (predicted).
N-glycosylated IL-7Ralpha binds IL7 300-fold more tightly than the unglycosylated form;Ubiquitinated by MARCHF8; leading to lysosomal degradation

Subunit：The IL7 receptor is a heterodimer of IL7R and IL2RG. The TSLP receptor is a heterodimer of CRLF2 and IL7R. Interacts with CD53 (PubMed:31748347)

Database

Entrez Gene: 3575 Human

SwissProt: P16871 Human

OMIM: 608971 Human

Research Field

Immunology

Synonyms

IL7R; Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CDw127; CD antigen CD127

Documentation

Select Batch:

Data Sheet (232 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

CD127 Antibody (YA545) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CD127 Antibody (YA545)

CD127 Antibody (YA545) Featured Recommendations:

Product Detail

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Background

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CD127 Antibody (YA545) Related Classifications

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