Cat. No.: HY-P80079

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CDK6 Antibody (YA511)

(Synonyms: CDKN6, CDK6, Cyclin-dependent kinase 6, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE)

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Based on 1 Customer Validation

CDK6 Antibody (YA511) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDK6.

For research use only. We do not sell to patients.

Host:
Rabbit
Isotype:
IgG
Application:
WB, IHC-P, ICC/IF, FC, IF-Tissue
Reactivity :
Human

Formulation:
Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Conjugation:
Non-conjugated

ALL WB IHC-P FC ICC

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Amount: USD 0.00

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications
Product Details
Product Properties
- Appearance
- Formulation
- Concentration
- Storage & Stability
- Shipping
Verification Images
Background
Documentation
References
Help & FAQs
Classifications
Bioz

Applications

Application	WB WB: Western Blot	ICC/IF ICC/IF: Immunocytochemistry/ Immunofluorescence	IF-Tissue IF-Tissue: Immunofluorescence-Tissue	IHC-P IHC-P: Immunohistochemistry-Paraffin	FC FC: Flow Cytometry
Dilution Ratio	1:500-1:2000	1:50-1:200	1:50-1:200	1:50-1:200	1:50-1:100

Product Details

Description

CDK6 Antibody (YA511) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDK6.

Host Rabbit
Clonality Recombinant,Monoclonal
Species Reactivity
Human
Observed Molecular Weight

Observed band size: 37 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 37 kDa

Species Reactivity Database

Entrez Gene: 1021 Human

SwissProt: Q00534 Human

OMIM: 616080 Human

Immunogen

Synthetic peptide corresponding to Human Cdk6.AA range:277-326.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102292

Product Properties

Appearance

Solution
Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration

Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Verification Images

Western blot analysis of extracts from Hela(lane 2(20μg) , K-562(lane 3(20μg) and HEK293(lane 4(20μg) using Cdk6 Antibody(HY-P80079) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature
Immunohistochemical analysis of paraffin-embedded human colon cancer using CDK6 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80079, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human ovarian cancer using CDK6 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80079, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human bladder cancer using CDK6 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80079, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human liver using CDK6 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80079, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human stomach cancer using CDK6 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80079, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human prostate cancer using CDK6 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80079, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human thyroid cancer using CDK6 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80079, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human tonsil using CDK6 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80079, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded human kidney using CDK6 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P80079, 1/200) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Flow cytometric analysis of 1X10^6 Hela cells labeling Cdk6 Antibody(HY-P80079, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Immunocytochemistry analysis of HepG2 cells labeling Cdk6 with Cdk6 Antibody (HY-P80079) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cdk6 Antibody (HY-P80079) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of Hela cells labeling Cdk6 with Cdk6 Antibody (HY-P80079) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Cdk6 Antibody (HY-P80079)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue)

Background

Function

CDK6 (cyclin-dependent kinase 6) is a serine/threonine kinase that forms active complexes with D-type cyclins and regulates G1-phase progression and the G1/S cell-cycle transition through phosphorylation of the retinoblastoma protein (RB)， thereby controlling E2F-dependent transcriptional programs^[1]^[2]. Mechanistically, CDK6 integrates mitogenic and hormone-derived signals through cyclin D activation, linking extracellular growth cues to cell-cycle entry and cellular proliferation^[1]^[3]. Beyond canonical cell-cycle regulation, accumulating evidence indicates that CDK6 contributes to tumor development and progression in hematologic malignancies and solid tumors, where dysregulated cyclin D-CDK6 signaling promotes uncontrolled proliferation and therapeutic resistance^[1]^[4]. In disease models of hormone receptor-positive breast cancer, aberrant CDK4/6 activity drives RB phosphorylation and cell-cycle progression, making this pathway a central therapeutic target^[3]^[5]. Compared with the closely related isoform CDK4, CDK6 displays distinct regulatory and biological functions, including kinase-dependent and kinase-independent activities that influence transcriptional regulation and tumor adaptation^[4]^[6]. For experimental applications, selective CDK4/6 inhibitors including palbociclib, ribociclib, and abemaciclib block the G1/S transition by suppressing CDK4/6 activity, although these compounds exhibit different affinities toward CDK4 and CDK6 and produce distinct biological and clinical effects^[3]^[5]. CDK6 overexpression, CDK6-containing resistant complexes, and alterations in RB-associated pathways have also been implicated in resistance mechanisms, supporting continued investigation of CDK6-selective targeting strategies in cancer research^[5]^[1].
Subcellular Localization

Cytoplasm; Nucleus; Cell projection, ruffle; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Expression

Tissue_specificity:Widely expressed. Accumulates in squamous cell carcinoma, proliferative hematopoietic progenitor cells, pancreatic β cells, and neuroblastoma. Levels are reduced in differentiated cells.

Induction:Down-regulated in response to enterovirus 71 (EV71) infection. Induced by NANOG during S-phase entry
Subunit

Interaction with D-type G1 cyclins. Cyclin binding promotes enzyme activation by phosphorylation at Thr-177 (By similarity). Binds to RUNX1, CDKN2D, FBXO7 and CDKN2C/p18-INK4c. Forms a cytoplasmic complex with Hsp90/HSP90AB1 and CDC37. FBXO7-binding promotes D-type cyclin binding. Interacts with Kaposi's sarcoma herpesvirus (KSHV) V-cyclin and herpesvirus saimiri (V-cyclin/ECLF2); the CDK6/V-cyclin complex phosphorylates NPM1 and thus lead to viral reactivation by reducing viral LANA levels
SwissProt ID

Q00534
Gene ID

1021 [NCBI]
Synonyms

CDKN6, CDK6, Cyclin-dependent kinase 6, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE
Research Field

Cell Biology