PKN1 Antibody (YA1664)
(Synonyms: DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA)PKN1 Antibody (YA1664) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PKN1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB
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Reactivity :
Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Conjugation:
Non-conjugated
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Applications
| Application |
WB
WB: Western Blot
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|---|---|
| Dilution Ratio | 1:500-1:1000 |
Product Details
PKN1 Antibody (YA1664) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PKN1.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityRat
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Observed Molecular WeightObserved band size: 120 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 104 kDa
Entrez Gene: 29355 Rat
SwissProt: Q63433 Rat
A synthetic peptide of human PKN
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PKN1 (protein kinase N1, PRK1) is a member of the protein kinase N family of AGC serine/threonine kinases that functions downstream of Rho family GTPases and phospholipid-dependent signaling to regulate cytoskeletal organization, cell migration, transcriptional control, and cell survival pathways[1][4]. Mechanistically, PKN1 links RhoA-dependent signaling to phosphorylation events that influence intermediate filament dynamics, actin-associated structures, and downstream signaling networks including PI3K/AKT-related processes[1][2]. Through these activities, PKN1 contributes to the regulation of cellular proliferation and differentiation, and genetic or functional perturbation of PKN1 alters signaling responses in multiple experimental systems[2][3]. In disease-relevant contexts, PKN1 has been associated with tumor cell invasion, androgen receptor-dependent transcriptional regulation, and cancer-related signaling pathways, supporting its use as a target for mechanistic studies of oncogenic signaling and cytoskeletal remodeling[4][5]. Compared with related isoforms, PKN1 and PKN2 are broadly expressed across tissues, whereas PKN3 displays a more restricted expression pattern and has been linked more specifically to tumor-associated functions, highlighting important isoform-specific biology within the PKN family[3]. For experimental applications, pharmacological kinase inhibitors and genetic loss-of-function approaches have been used to define PKN1-dependent signaling networks, enabling investigation of isoform-selective functions and evaluation of PKN1 as a potential therapeutic target in cancer and other disease models[2][3].
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Subcellular Localization
Cytoplasm; Nucleus; Endosome; Cell membrane; Peripheral membrane protein; Cleavage furrow; Midbody
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Expression
Tissue_specificity:It is widely present in various tissues. It is expressed in the heart, brain, placenta, lungs, skeletal muscle, kidneys, and pancreas. It is also expressed in a variety of tumor cell lines, especially breast tumor cells. -
Isoforms & Post-Translational Modification
Q16512 has 3 isomers: Q16512-1: 103932 Da (predicted); Q16512-2: 104673 Da (predicted); Q16512-3: 66060 Da (predicted).
Autophosphorylated; preferably on serine. Phosphorylated during mitosis;Activated by limited proteolysis with trypsin;(Microbial infection) In case of infection, polyubiquitinated by the bacterial E3 ubiquitin-protein ligase SspH1, leading to its proteasomal degradation -
Subunit
Interacts with ZFAND6 (By similarity). Interacts with AR (PubMed:12514133). Interacts with PRKCB (PubMed:20228790). Interacts (via REM 1 and REM 2 repeats) with RAC1 (PubMed:14514689, PubMed:18006505). Interacts (via REM 1 repeat) with RHOA (PubMed:10619026, PubMed:8571126). Interacts with RHOB (PubMed:9478917). Interacts (via C-terminus) with PDPK1 (PubMed:10792047). Interacts with CCNT2; enhances MYOD1-dependent transcription (PubMed:16331689). Component of a signaling complex containing at least AKAP13, PKN1, MAPK14, ZAK and MAP2K3. Within this complex, AKAP13 interacts directly with PKN1, which in turn recruits MAPK14, MAP2K3 and ZAK (PubMed:21224381)
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SwissProt ID
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Synonyms
DBK; PKN; PAK1; PRK1; PAK-1; PRKCL1; PKN-ALPHA
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Research Field
Signal Transduction
Documentation
References
[1]. Uniprotkb.
[2]. Yasui T, et al. Protein kinase N1, a cell inhibitor of Akt kinase, has a central role in quality control of germinal center formation. Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):21022-7. [Content Brief]
[3]. Falk MD, et al. Enzyme kinetics and distinct modulation of the protein kinase N family of kinases by lipid activators and small molecule inhibitors. Biosci Rep. 2014;34(2):e00097. [Content Brief]
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