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CaMKII gamma Antibody (YA4248)

(Synonyms: CAMK; CAMKG; CAMK-II)
Customer Review

Based on 1 Customer Validation

CaMKII gamma Antibody (YA4248) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CAMK2 gamma.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse, Rat, Monkey, Rabbit

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • CaMKII gamma Antibody (YA4248) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:100-1:500 1:200-1:400 1:10000

Product Details

Description

CaMKII gamma Antibody (YA4248) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CAMK2 gamma.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat, Monkey, Rabbit
  • Observed Molecular Weight
    Observed band size: 63 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 63 kDa
Immunogen

Purified recombinant fragment of human CAMK2G (AA: 322-481) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3718934

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    Western blot analysis of extracts from Jurkat (lane 2(20μg), HepG2 (lane 3(20μg), C2C12 (lane 4(20μg), C6 (lane 5(20μg), using CAMK2 gamma Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    Western blot analysis of extracts from K562(lane 1(40μg) )
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    Immunohistochemical analysis of paraffin-embedded human pancreas using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    Immunohistochemical analysis of paraffin-embedded human lung tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    Immunohistochemical analysis of paraffin-embedded human spleen tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    Immunohistochemical analysis of paraffin-embedded human pancreas using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    Immunohistochemical analysis of paraffin-embedded human stomach tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    Immunohistochemical analysis of paraffin-embedded human lung tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    Immunohistochemical analysis of paraffin-embedded human spleen tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    Flow cytometric analysis of 1X10^6 Jurkat cells labeling CAMK2 gamma Antibody (HY-P84551, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    CAMK2 gamma is a Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in sarcoplasmic reticulum Ca(2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR tH2O2gh phosphorylation of the ryanodine receptor-coupling factor triadin. In the central nervous system, it is involved in the regulation of neurite formation and arborization. It may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway[1][2].

  • Subcellular Localization

    Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side

  • Expression


    Tissue_specificity:Expressed in skeletal muscle

    Induction:Activity is induced in skeletal muscle during exercise

  • Subunit

    CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D).

  • SwissProt ID

    Q13555

  • Gene ID
    818 [NCBI]
  • Synonyms

    CAMK; CAMKG; CAMK-II

CaMKII gamma Antibody (YA4248) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), HepG2 (lane 3(20μg), C2C12 (lane 4(20μg), C6 (lane 5(20μg), using CAMK2 gamma Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 1(40μg) )

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Jurkat cells labeling CAMK2 gamma Antibody (HY-P84551, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CaMKII gamma Antibody (YA4248) Product Image
    1/16
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • CaMKII gamma Antibody (YA4248) Product Image
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Jurkat (lane 2(20μg), HepG2 (lane 3(20μg), C2C12 (lane 4(20μg), C6 (lane 5(20μg), using CAMK2 gamma Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562(lane 1(40μg) )
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lung tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Jurkat cells labeling CAMK2 gamma Antibody (HY-P84551, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • Experimental Validation Results for CaMKII gamma Antibody (YA4248)
  • CaMKII gamma Antibody (YA4248) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Jurkat (lane 2(20μg), HepG2 (lane 3(20μg), C2C12 (lane 4(20μg), C6 (lane 5(20μg), using CAMK2 gamma Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • CaMKII gamma Antibody (YA4248) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562(lane 1(40μg) )

  • CaMKII gamma Antibody (YA4248) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreas using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CaMKII gamma Antibody (YA4248) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CaMKII gamma Antibody (YA4248) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CaMKII gamma Antibody (YA4248) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CaMKII gamma Antibody (YA4248) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CaMKII gamma Antibody (YA4248) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreas using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CaMKII gamma Antibody (YA4248) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human stomach tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CaMKII gamma Antibody (YA4248) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CaMKII gamma Antibody (YA4248) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lung tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CaMKII gamma Antibody (YA4248) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using CAMK2 Antibody (HY-P84551, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CaMKII gamma Antibody (YA4248) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Jurkat cells labeling CAMK2 gamma Antibody (HY-P84551, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • CaMKII gamma Antibody (YA4248) Product Image WB
  • CaMKII gamma Antibody (YA4248) Product Image WB
  • CaMKII gamma Antibody (YA4248) Product Image IHC-P
  • CaMKII gamma Antibody (YA4248) Product Image IHC-P
  • CaMKII gamma Antibody (YA4248) Product Image IHC-P
  • CaMKII gamma Antibody (YA4248) Product Image IHC-P
  • CaMKII gamma Antibody (YA4248) Product Image IHC-P
  • CaMKII gamma Antibody (YA4248) Product Image IHC-P
  • CaMKII gamma Antibody (YA4248) Product Image IHC-P
  • CaMKII gamma Antibody (YA4248) Product Image IHC-P
  • CaMKII gamma Antibody (YA4248) Product Image IHC-P
  • CaMKII gamma Antibody (YA4248) Product Image IHC-P
  • CaMKII gamma Antibody (YA4248) Product Image FC