phospho-TAOK 1/2/3 (S181/S181/S177) Antibody (YA7250)

Cat. No.: HY-P87566: Data Sheet User Guide for Antibodies
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phospho-TAOK 1/2/3 (S181/S181/S177) Antibody (YA7250) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to phospho-TAOK 1/2/3 (S181/S181/S177).

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

phospho-TAOK 1/2/3 (S181/S181/S177) Antibody (YA7250) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to phospho-TAOK 1/2/3 (S181/S181/S177).

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 116 kDa;

Observed band size: 110130140 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human Predicted Reactivity: Mouse,Rat

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

Q9UL54

Immunogen

A synthesized peptide derived from human TAOK 1/2/3 around the phosphorylation site of S181/S181/S177

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-200
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-200
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:100-200

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Serine/threonine-protein kinase involved in different processes such as membrane blebbing and apoptotic bodies formation DNA damage response and MAPK14/p38 MAPK stress-activated MAPK cascade. Phosphorylates itself, MBP, activated MAPK8, MAP2K3, MAP2K6 and tubulins. Activates the MAPK14/p38 MAPK signaling pathway through the specific activation and phosphorylation of the upstream MAP2K3 and MAP2K6 kinases. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Isoform 1, but not isoform 2, plays a role in apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation. This function, which requires the activation of MAPK8/JNK and nuclear localization of C-terminally truncated isoform 1, may be linked to the mitochondrial CASP9-associated death pathway. Isoform 1 binds to microtubules and affects their organization and stability independently of its kinase activity. Prevents MAP3K7-mediated activation of CHUK, and thus NF-kappa-B activation, but not that of MAPK8/JNK. May play a role in the osmotic stress-MAPK8 pathway. Isoform 2, but not isoform 1, is required for PCDH8 endocytosis. Following homophilic interactions between PCDH8 extracellular domains, isoform 2 phosphorylates and activates MAPK14/p38 MAPK which in turn phosphorylates isoform 2. This process leads to PCDH8 endocytosis and CDH2 cointernalization. Both isoforms are involved in MAPK14 phosphorylation

Subcellular Localization：Cytoplasmic vesicle membrane,Cytoplasm, cytoskeleton,Nucleus,Cell projection, dendrite

Expression：
Tissue_Specificity: Ubiquitously expressed, with a higher level of expression in testis and brain

Isoforms & Post-Translational Modification：Q9UL54 has four isomers: Q9UL54-1: 138251 Da (predicted); Q9UL54-2: 119281 Da (predicted); Q9UL54-3: 118780 Da (predicted); Q9UL54-4: 126204 Da (predicted).
Isoforms 1 and 2 are autophosphorylated丨C-terminal cleavage of isoform 1 and subsequent nuclear localization requires CASP9 activity丨Autophosphorylated丨Phosphorylated on Ser-1031 by MAPK14

Subunit：Interacts with MAP2K3 and MAP2K6 (By similarity)

각종 서류

User Guide for Antibodies (1077 KB)