GAP43 Antibody (YA5421)

Cat. No.: HY-P85729: Data Sheet User Guide for Antibodies
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GAP43 Antibody (YA5421) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to GAP43.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 weeks 1 - 2 Weeks 3 - 4 weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

GAP43 Antibody (YA5421) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to GAP43.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Observed band size: 38,43 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P17677

Gene ID

2596 [NCBI]

Immunogen

Recombinant Protein of GAP-43

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-200

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：This protein is associated with nerve growth. It is a major component of the motile 'growth cones' that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction

Subcellular Localization：Cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side; Synapse; Cell projection, filopodium membrane; Peripheral membrane protein; Perikaryon; Cell projection, dendrite; Cell projection, axon; Cytoplasm

Isoforms & Post-Translational Modification：P17677 has 2 isomers: P17677-1: 24803 Da (predicted); P17677-2: 28766 Da (predicted).
Phosphorylated (By similarity). Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity (By similarity);Palmitoylated by ZDHHC3 (By similarity). Palmitoylation is regulated by ARF6 and is essential for plasma membrane association and axonal and dendritic filopodia induction (PubMed:14978216). Deacylated by LYPLA2 (PubMed:21152083)

Subunit：Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN (By similarity). Interacts (via IQ domain) with calmodulin (By similarity). Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence (By similarity)

Synonyms

GAP43; Neuromodulin; Axonal membrane protein GAP-43; Growth-associated protein 43; Neural phosphoprotein B-50; pp46

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/pc-3-cell.html [Content Brief]