GLO1 Antibody (YA9734)
(Synonyms: Aldoketomutase, glo1, GLOD1, Glx I, GLYI, glyoxalase domain containing 1, Glyoxalase I, Ketone aldehyde mutase, Ketone-aldehyde mutase, Lactoyl glutathione lyase)GLO1 Antibody (YA9734) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to GLO1.
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB, ICC/IF, IHC-P, FC
-
Reactivity :
Human, Mouse, Rat
-
Formulation:
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-500 | 1:100-500 |
Product Details
GLO1 Antibody (YA9734) is a Rabbit-derived and non-conjugated IgG Recombinant,Monoclonal antibody, targeting to GLO1.
-
Host Rabbit
-
Clonality Recombinant,Monoclonal
-
Species ReactivityHuman, Mouse, Rat
-
Calculated Molecular Weight Predicted band size: 21 kDa;
Recombinant protein of Human GLO1 (aa 1-120).
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
GLO1 catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis (By similarity)[1][2][3][4].
-
Isoforms & Post-Translational Modification
GLO1 has 2 isoforms, Q04760-1: amino acid length is 184, molecular weight is 20778 Da (predicted); Q04760-2: amino acid length is 169, molecular weight is 19043 Da (predicted).
Glutathionylation at Cys-139 inhibits enzyme activity. -
Subunit
Homodimer
-
SwissProt ID
-
Synonyms
Aldoketomutase, glo1, GLOD1, Glx I, GLYI, glyoxalase domain containing 1, Glyoxalase I, Ketone aldehyde mutase, Ketone-aldehyde mutase, Lactoyl glutathione lyase
Documentation
References
[1]. Birkenmeier G, et al. Posttranslational modification of human glyoxalase 1 indicates redox-dependent regulation. PLoS One. 2010 Apr 29;5(4):e10399. [Content Brief]
[2]. Chiba T, et al. Design and evaluation of azaindole-substituted N-hydroxypyridones as glyoxalase I inhibitors. Bioorg Med Chem Lett. 2012 Dec 15;22(24):7486-9. [Content Brief]
[3]. Ridderström M, et al. Involvement of an active-site Zn2+ ligand in the catalytic mechanism of human glyoxalase I. J Biol Chem. 1998 Aug 21;273(34):21623-8. [Content Brief]
[4]. de Hemptinne V, et al. Phosphorylation on Thr-106 and NO-modification of glyoxalase I suppress the TNF-induced transcriptional activity of NF-kappaB. Mol Cell Biochem. 2009 May;325(1-2):169-78. [Content Brief]
Powered by Bioz
