Lambda Light Chain Antibody (YA6642)

Cat. No.: HY-P86949: Data Sheet User Guide for Antibodies
FAQs
Technical Support

Lambda Light Chain Antibody (YA6642) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Lambda Light Chain.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Lambda Light Chain Antibody (YA6642) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Lambda Light Chain.

Host

Mouse

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 11 kDa;

Observed band size: 25 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

P0CG04

Gene ID

Immunogen

Constant region of natural protein (constant region of light chain lambda chain).

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:4000
FC FC: Flow Cytometry	1:1000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268)

Subcellular Localization：Secreted,Cell membrane

Isoforms & Post-Translational Modification：P0CG04: 106 amino acids, molecular weight 11348 Da.
Proteolytically cleaved at the N-terminus

Subunit：Immunoglobulins are composed of two identical heavy chains and two identical light chains; disulfide-linked

Synonyms

Constant region of lambda light chains antibody; Ig lambda chain C regions antibody; IGLC antibody; Immunoglobulin lambda constant 1 antibody; Mcg marker antibody;

Documentation

User Guide for Antibodies (1077 KB)