Phospho-STAT5(Tyr694) Antibody (YA1513)

Cat. No.: HY-P81768: Data Sheet COA
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User Guide for Antibodies
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Phospho-STAT5(Tyr694) Antibody (YA1513) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-STAT5(Tyr694).

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사이즈	재고	수량
20 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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Phospho-STAT5(Tyr694) Antibody (YA1513) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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Phospho-STAT5(Tyr694) Antibody (YA1513) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-STAT5(Tyr694).

Host

Rabbit

Clonality

Recombinant, Monoclonal

분자량

Predicted band size: 91 kDa;

Observed band size: 91 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P42229

Gene ID

6776 [NCBI]

Immunogen

A synthesized peptide derived from human Phospho-Stat5 (Y694) DG-pY-VK.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from A431 (lane 2(20μg), A431 (lane 3(40μg), using Phospho-STAT5(Tyr694) Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation

Subcellular Localization：Cytoplasm; Nucleus

Isoforms & Post-Translational Modification：P42229 has 2 isomers: P42229-1: 90647 Da (predicted); P42229-2: 86949 Da (predicted).
Tyrosine phosphorylated in response to KITLG/SCF, IL2, IL3, IL7, IL15, CSF2/GMCSF, GH1, PRL, EPO and THPO (By similarity). Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus (PubMed:21135090). Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity (By similarity). Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3 (PubMed:14504097). Alternatively, can be phosphorylated by JAK2 at Tyr-694 (PubMed:12529425);ISGylated

Subunit：Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1 and SOCS7 (PubMed:12954634). Interacts with ERBB4 (PubMed:15534001). Interacts with EBF4 (PubMed:35939714). Interacts with CD69 (By similarity)

RRID

AB_3103874

Database

Entrez Gene: 6776 Human ;

SwissProt: P42229 Human ; P51692 Human ;

OMIM: 601511 Human

Research Field

Signal Transduction

Synonyms

STAT5A; STAT5; Signal transducer and activator of transcription 5A

각종 서류

Select Batch:

Data Sheet (232 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Phospho-STAT5(Tyr694) Antibody (YA1513) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phospho-STAT5(Tyr694) Antibody (YA1513)

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Phospho-STAT5(Tyr694) Antibody (YA1513) Related Classifications

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