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PHD1 Antibody (YA7262)

(Synonyms: DKFZp434E026 antibody; Egl nine homolog 2 antibody; EGLN 2 antibody; EIT 6 antibody; Estrogen-induced tag 6 antibody; HIF P4H 1 antibody; HIF prolyl hydroxylase 1 antibody; HIF-PH1 antibody; HIF-prolyl hydroxylase 1 antibody; HIFPH 1 antibody; HPH-3 antibody; HPH3 antibody; Hypoxia-inducible factor prolyl hydroxylase 1 antibody; P4H1 antibody; PHD 1 antibody; prolyl hydroxylase domain containing protein 1 antibody )
Customer Review

Based on 1 Customer Validation

PHD1 Antibody (YA7262) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHD1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC, IF-Tissue

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for PHD1 Antibody (YA7262)
  • Experimental Validation Results for PHD1 Antibody (YA7262)
  • Experimental Validation Results for PHD1 Antibody (YA7262)
  • PHD1 Antibody (YA7262) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
FC Info
FC: Flow Cytometry
Dilution Ratio 1:1000-5000 1:1000 1:50-1:200 1:50-200 1:50-100

Product Details

Description

PHD1 Antibody (YA7262) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PHD1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 44 kDa
Immunogen

A synthesized peptide derived from human PHD1

Sensitivity

Endogenous

Purification

affinity purified by Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for PHD1 Antibody (YA7262)
    Western blot analysis of extracts from L929 (lane 2(20μg), NIH/3T3 (lane 3(20μg), HCT116 (lane 4(20μg), HEK293T (lane 5(20μg) using PHD1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PHD1 Antibody (YA7262)
    Immunocytochemistry analysis of A549 cells labeling PHD1 Antibody with PHD1 Antibody (YA7262) (HY-P87578) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHD1 Antibody (YA7262) ((HY-P87578) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PHD1 Antibody (YA7262)
    Immunocytochemistry analysis of Hela cells labeling PHD1 Antibody with PHD1 Antibody (YA7262) (HY-P87578) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHD1 Antibody (YA7262) ((HY-P87578) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    PHD1 is a HIF prolyl hydroxylase that regulates HIF-1 protein levels in mammalian cells. HIF-1α prolyl hydroxylases mainly include PHD1, PHD2, and PHD3. These three enzymes have sequence-similar catalytic domains and conserve amino acid residues for coordinating Fe (II) ions. Under hypoxic conditions, due to insufficient oxygen supply, the hydroxylase activity of PHD1 decreases, leading to HIF-1α accumulation and inducing the expression of HRE-regulated target genes. Therefore, PHDs are considered intracellular oxygen sensors. PHD1 is found only in the cell nucleus, while PHD2 is mainly located in the cytoplasm.

  • Subcellular Localization

    Nucleus

  • Expression


    Tissue_Specificity: Expressed in adult and fetal heart, brain, liver, lung, skeletal muscle, and kidney. Also expressed in testis and placenta. Highest levels in adult brain, placenta, lung, kidney, and testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium
    Induction: By estrogen (PubMed:11850811). Induced by proteasomal inhibitor MG132 (at protein level) (PubMed:16509823)|Induced by hypoxia leading to protein stability|Repressed by hypoxia

  • Isoforms & Post-Translational Modification

    Q96KS0 has two isomers: Q96KS0-1: 43650 Da (predicted); Q96KS0-2: 40168 Da (predicted).
    Ubiquitinated by SIAH1 and/or SIAH2 in response to the unfolded protein response (UPR), leading to its degradation

  • Subunit

    Interacts (preferably isoform p40) with SIAH2; the interaction targets both SIAH2 isoforms for proteasomal degradation in vitro (PubMed:16509823)

  • SwissProt ID

    Q96KS0

  • Synonyms

    DKFZp434E026 antibody; Egl nine homolog 2 antibody; EGLN 2 antibody; EIT 6 antibody; Estrogen-induced tag 6 antibody; HIF P4H 1 antibody; HIF prolyl hydroxylase 1 antibody; HIF-PH1 antibody; HIF-prolyl hydroxylase 1 antibody; HIFPH 1 antibody; HPH-3 antibody; HPH3 antibody; Hypoxia-inducible factor prolyl hydroxylase 1 antibody; P4H1 antibody; PHD 1 antibody; prolyl hydroxylase domain containing protein 1 antibody

[1]. Yasumoto K, et al. Role of the intracellular localization of HIF-prolyl hydroxylases. Biochim Biophys Acta. 2009 May;1793(5):792-7. [Content Brief]

PHD1 Antibody (YA7262) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from L929 (lane 2(20μg), NIH/3T3 (lane 3(20μg), HCT116 (lane 4(20μg), HEK293T (lane 5(20μg) using PHD1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling PHD1 Antibody with PHD1 Antibody (YA7262) (HY-P87578) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHD1 Antibody (YA7262) ((HY-P87578) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PHD1 Antibody with PHD1 Antibody (YA7262) (HY-P87578) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHD1 Antibody (YA7262) ((HY-P87578) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PHD1 Antibody (YA7262) Product Image
    1/16
  • Experimental Validation Results for PHD1 Antibody (YA7262)
  • Experimental Validation Results for PHD1 Antibody (YA7262)
  • Experimental Validation Results for PHD1 Antibody (YA7262)
  • PHD1 Antibody (YA7262) Product Image
  • Experimental Validation Results for PHD1 Antibody (YA7262)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from L929 (lane 2(20μg), NIH/3T3 (lane 3(20μg), HCT116 (lane 4(20μg), HEK293T (lane 5(20μg) using PHD1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for PHD1 Antibody (YA7262)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling PHD1 Antibody with PHD1 Antibody (YA7262) (HY-P87578) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHD1 Antibody (YA7262) ((HY-P87578) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PHD1 Antibody (YA7262)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling PHD1 Antibody with PHD1 Antibody (YA7262) (HY-P87578) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHD1 Antibody (YA7262) ((HY-P87578) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for PHD1 Antibody (YA7262)
  • Experimental Validation Results for PHD1 Antibody (YA7262)
  • Experimental Validation Results for PHD1 Antibody (YA7262)
  • PHD1 Antibody (YA7262) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from L929 (lane 2(20μg), NIH/3T3 (lane 3(20μg), HCT116 (lane 4(20μg), HEK293T (lane 5(20μg) using PHD1 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • PHD1 Antibody (YA7262) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling PHD1 Antibody with PHD1 Antibody (YA7262) (HY-P87578) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHD1 Antibody (YA7262) ((HY-P87578) at 1/50 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PHD1 Antibody (YA7262) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling PHD1 Antibody with PHD1 Antibody (YA7262) (HY-P87578) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with PHD1 Antibody (YA7262) ((HY-P87578) at 1/100 dilution in BSA for Immunol Staining at 4 ℃, Stay overnight. AF488-conjugated AffiniPure Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • PHD1 Antibody (YA7262) Product Image WB
  • PHD1 Antibody (YA7262) Product Image ICC
  • PHD1 Antibody (YA7262) Product Image ICC