TGN46 Antibody (YA3228)

(Synonyms: TGN38; TGN46; TGN48; TGN51; TGOLN2; TTGN2)
Customer Review

Based on 1 Customer Validation

TGN46 Antibody (YA3228) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TGN46.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IP

  • Reactivity :

    Human

  • Formulation:

    Supplied in rabbit IgG in 1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000-1:20000 1:100-1:4000 1:50

Product Details

Description

TGN46 Antibody (YA3228) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TGN46.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 75-110 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 46 kDa
Species Reactivity Database

Entrez Gene: 10618 Human

SwissProt: O43493 Human

Immunogen

A synthesized peptide derived from human TGN46 aa20-75/437.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TGN46 Antibody (YA3228)
    Immunohistochemical analysis of paraffin-embedded human colon cacner using TGOLN2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83483, 1/4000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TGN46 Antibody (YA3228)
    Immunohistochemical analysis of paraffin-embedded human pancreas using TGOLN2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83483, 1/4000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TGN46 Antibody (YA3228)
    Immunohistochemical analysis of paraffin-embedded human ovarian cacner using TGOLN2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83483, 1/4000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TGN46 Antibody (YA3228)
    Immunohistochemical analysis of paraffin-embedded human liver cacner using TGOLN2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83483, 1/4000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TGN46 Antibody (YA3228)
    Immunohistochemical analysis of paraffin-embedded human prostate using TGOLN2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83483, 1/4000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TGN46 Antibody (YA3228)
    Immunohistochemical analysis of paraffin-embedded human liver using TGOLN2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83483, 1/4000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TGN46 Antibody (YA3228)
    Immunohistochemical analysis of paraffin-embedded human kidney using TGOLN2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83483, 1/4000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for TGN46 Antibody (YA3228)
    Immunohistochemical analysis of paraffin-embedded human head and neck cacner using TGOLN2 antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83483, 1/4000) for 30 minutes at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    TGOLN2 may be involved in regulating membrane traffic to and from trans-Golgi network

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:Isoform TGN46 is widely expressed. Isoform TGN51 is more abundant in fetal lung and kidney. Isoform TGN48 is barely expressed in embryonic kidney and promyelocytic cells

  • Isoforms & Post-Translational Modification

    O43493 has 6 isomers: O43493-2: 45880 Da (predicted); O43493-3: 47698 Da (predicted); O43493-4: 39563 Da (predicted); O43493-5: 47058 Da (predicted); O43493-6: 29541 Da (predicted); O43493-7: 47880 Da (predicted).

  • SwissProt ID

    O43493

  • Gene ID
  • Synonyms

    TGN38; TGN46; TGN48; TGN51; TGOLN2; TTGN2

  • Research Field

    Tags & Cell Markers

TGN46 Antibody (YA3228) Related Classifications

MOQ
Minimum order quantity
100 mg

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