MST3 Antibody (YA6978)

Cat. No.: HY-P87295: Data Sheet User Guide for Antibodies
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MST3 Antibody (YA6978) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MST3.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MST3 Antibody (YA6978) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MST3.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 50/35 kDa;

Observed band size: 50/35 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9Y6E0

Immunogen

Amino acids 275-393 of MST-3 of human origin.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:100-1:1000
IP IP: Immunoprecipitation	1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:500

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.

Storage & Stability

Stored at 2-8°C for 1 year, do not freeze.

Shipping

Shipping with blue ice.

Background

Function：Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. In association with STK26 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation (PubMed:27807006). Also regulates cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed:18782753)

Subcellular Localization：Cytoplasm,Nucleus,Membrane

Expression：
Tissue_Specificity: Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex

Isoforms & Post-Translational Modification：Q9Y6E0 has two isomers: Q9Y6E0-1: 49308 Da (predicted); Q9Y6E0-2: 47913 Da (predicted).
Proteolytically processed by caspases during apoptosis丨Isoform B is activated by phosphorylation by PKA

Subunit：Monomer (PubMed:20124694)

Synonyms

epididymis secretory protein Li 95 antibody; HEL-S-95 antibody; Mammalian STE20 like protein kinase 3 antibody; Mammalian STE20-like protein kinase 3 antibody; Mammalian STE20-like protein kinase 3 C-terminal antibody; Mammalian STE20-like protein kinase 3 N-terminal antibody; Mammalian sterile 20-like 3 antibody; MST-3 antibody; MST3/C antibody; MST3/N antibody

Documentation

User Guide for Antibodies (1077 KB)