MST3 Antibody (YA6978)
(Synonyms: epididymis secretory protein Li 95 antibody; HEL-S-95 antibody; Mammalian STE20 like protein kinase 3 antibody; Mammalian STE20-like protein kinase 3 antibody; Mammalian STE20-like protein kinase 3 C-terminal antibody; Mammalian STE20-like protein kinase 3 N-terminal antibody; Mammalian sterile 20-like 3 antibody; MST-3 antibody; MST3/C antibody; MST3/N antibody)MST3 Antibody (YA6978) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MST3.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, ICC/IF, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
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| Dilution Ratio | 1:100-1:1000 | 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) | 1:50-1:500 |
Product Details
MST3 Antibody (YA6978) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MST3.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 50/35 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 50/35 kDa
Amino acids 275-393 of MST-3 of human origin.
Endogenous
Protein A affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
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Storage & Stability
Stored at 2-8°C for 1 year, do not freeze.
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Shipping
Shipping with blue ice.
Background
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Function
MST3 (STK24) is a serine/threonine kinase in the germinal-center kinase III family, with an N-terminal kinase domain and C-terminal regulatory domain[1]. Mechanistically, caspase cleavage activates MST3 kinase activity, promotes nuclear translocation, and induces apoptotic morphology in Jurkat-cell apoptosis models[2]. MST3 also phosphorylates NDR1/2 at hydrophobic-motif sites and supports G1/S cell-cycle progression through the NDR-p21 axis[3][4]. In neural models, MST3 phosphorylates TAO1/2 to enable Myosin Va-dependent dendritic localization and excitatory synapse development[5]. Cdk5-dependent MST3 phosphorylation regulates neuronal migration by inhibiting RhoA activity[6]. In immune-cell models, the CCM3-STK24 complex coordinates UNC13D-driven vesicle exocytosis in neutrophils[7]. Compared with related isoforms, MST3/STK24 differs from MST1/2 in experimental inhibitor response, because MST3/4-selective inhibition causes G1 arrest, whereas MST1/2 inhibition causes G2/M accumulation[8]. For experimental applications, MR24 and MR30 provide selective MST3/4 chemical tools for dissecting MST3-dependent cell-cycle biology[8].- MST3 connects apoptosis, cell-cycle control, neuronal migration, synapse development, and neutrophil exocytosis.- MST3 differs from MST1/2 through distinct inhibitor-driven cell-cycle arrest phenotypes.- MST3/4 inhibitors support pathway dissection, but disease-translation claims require model-specific validation.
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Subcellular Localization
Cytoplasm,Nucleus,Membrane
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Expression
Tissue_Specificity: Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex -
Isoforms & Post-Translational Modification
Q9Y6E0 has two isomers: Q9Y6E0-1: 49308 Da (predicted); Q9Y6E0-2: 47913 Da (predicted).
Proteolytically processed by caspases during apoptosis丨Isoform B is activated by phosphorylation by PKA -
Subunit
Monomer (PubMed:20124694)
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SwissProt ID
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Synonyms
epididymis secretory protein Li 95 antibody; HEL-S-95 antibody; Mammalian STE20 like protein kinase 3 antibody; Mammalian STE20-like protein kinase 3 antibody; Mammalian STE20-like protein kinase 3 C-terminal antibody; Mammalian STE20-like protein kinase 3 N-terminal antibody; Mammalian sterile 20-like 3 antibody; MST-3 antibody; MST3/C antibody; MST3/N antibody
Documentation
[1]. Schinkmann K, et al. Cloning and characterization of a human STE20-like protein kinase with unusual cofactor requirements. J Biol Chem. 1997 Nov 7;272(45):28695-703. [Content Brief]
[2]. Huang CY, et al. Caspase activation of mammalian sterile 20-like kinase 3 (Mst3). Nuclear translocation and induction of apoptosis. J Biol Chem. 2002 Sep 13;277(37):34367-74. [Content Brief]
[3]. Hromatka BS, et al. Transcriptional response of Candida albicans to nitric oxide and the role of the YHB1 gene in nitrosative stress and virulence. Mol Biol Cell. 2005 Oct;16(10):4814-26. [Content Brief]
[4]. Cornils H, et al. Human NDR kinases control G(1)/S cell cycle transition by directly regulating p21 stability. Mol Cell Biol. 2011 Apr;31(7):1382-95. [Content Brief]
[5]. Ultanir SK, et al. MST3 kinase phosphorylates TAO1/2 to enable Myosin Va function in promoting spine synapse development. Neuron. 2014 Dec 3;84(5):968-82. [Content Brief]
[6]. Tang J, et al. Cdk5-dependent Mst3 phosphorylation and activity regulate neuronal migration through RhoA inhibition. J Neurosci. 2014 May 28;34(22):7425-36. [Content Brief]
[7]. Zhang Y, et al. A network of interactions enables CCM3 and STK24 to coordinate UNC13D-driven vesicle exocytosis in neutrophils. Dev Cell. 2013 Oct 28;27(2):215-226. [Content Brief]
[8]. Rak M, et al. Development of Selective Pyrido[2,3-d]pyrimidin-7(8H)-one-Based Mammalian STE20-Like (MST3/4) Kinase Inhibitors. J Med Chem. 2024 Mar 14;67(5):3813-3842. [Content Brief]
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