Phospho-MITF (Ser180) Antibody (YA9985)
(Synonyms: BHLHE32, MITF, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32)Phospho-MITF (Ser180) Antibody (YA9985) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to Phospho-MITF (Ser180).
-
Host:
Rabbit
-
Isotype:
IgG
-
Application:
WB
-
Reactivity :
Human, Mouse, Rat
-
Formulation:
Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA and 0.01% sodium azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
|
|---|---|
| Dilution Ratio | 1:1000-2000 |
Product Details
Phospho-MITF (Ser180) Antibody (YA9985) is a Rabbit-derived and non-conjugated IgG Monoclonal, Recombinant antibody, targeting to Phospho-MITF (Ser180).
-
Host Rabbit
-
Clonality Monoclonal,Recombinant
-
Species ReactivityHuman, Mouse, Rat
-
Observed Molecular WeightObserved band size: 57 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
-
Calculated Molecular Weight Predicted band size: 58, 57, 55, 46, 40 kDa
Synthetic phosphopeptide corresponding to residues surrounding S180 of human MITF protein.
Endogenous
affinity purified.
Non-conjugated
Phosphorylated
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in PBS (pH 7.4), containing 50% glycerol, 0.05% BSA and 0.01% sodium azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
MITF is a Transcription factor that acts as a master regulator of melanocyte survival and differentiation as well as melanosome biogenesis. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoter of pigmentation genes, such as tyrosinase (TYR). Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, MITF phosphorylation by MTOR promotes its inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces MITF dephosphorylation, resulting in transcription factor activity. Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium[1][2][3][4][5].
-
Subcellular Localization
Nucleus; Cytoplasm; Lysosome membrane
-
Expression
Tissue_Specificity: Expressed in melanocytes (at protein level). -
Isoforms & Post-Translational Modification
Phospho-MITF has 12 isoforms, O75030-1: amino acid length is 526, molecular weight is 58795 Da (predicted); O75030-2: amino acid length is 520, molecular weight is 58163 Da (predicted); O75030-3: amino acid length is 501, molecular weight is 56038 Da (predicted); O75030-4: amino acid length is 495, molecular weight is 55405 Da (predicted); O75030-5: amino acid length is 525, molecular weight is 58664 Da (predicted); O75030-6: amino acid length is 519, molecular weight is 58031 Da (predicted); O75030-7: amino acid length is 510, molecular weight is 57045 Da (predicted); O75030-8: amino acid length is 504, molecular weight is 56412 Da (predicted); O75030-9: amino acid length is 419, molecular weight is 46938 Da (predicted); O75030-10: amino acid length is 413, molecular weight is 46305 Da (predicted); O75030-11: amino acid length is 357, molecular weight is 40365 Da (predicted); O75030-12: amino acid length is 468, molecular weight is 52527 Da (predicted).
When nutrients are present, phosphorylation by MTOR at Ser-5 via non-canonical mTORC1 pathway promotes ubiquitination by the SCF(BTRC) complex, followed by degradation. Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter. Phosphorylation by MARK3/cTAK1 at Ser-280 promotes association with 14-3-3/YWHA adapters and retention in the cytosol. Phosphorylated at Ser-180 and Ser-516 following KIT signaling, triggering a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome. Phosphorylated in response to blue light (415nm). -
Subunit
Homodimer or heterodimer; dimerization is mediated via the coiled coil region.
-
SwissProt ID
-
Synonyms
BHLHE32, MITF, Microphthalmia-associated transcription factor, Class E basic helix-loop-helix protein 32, bHLHe32
Documentation
References
[1]. Takeda K, et al. Ser298 of MITF, a mutation site in Waardenburg syndrome type 2, is a phosphorylation site with functional significance. Hum Mol Genet. 2000 Jan 1;9(1):125-32. [Content Brief]
[2]. Genovese G, et al. The tumor suppressor HINT1 regulates MITF and β-catenin transcriptional activity in melanoma cells. Cell Cycle. 2012 Jun 1;11(11):2206-15. [Content Brief]
[3]. George A, et al. Biallelic Mutations in MITF Cause Coloboma, Osteopetrosis, Microphthalmia, Macrocephaly, Albinism, and Deafness. Am J Hum Genet. 2016 Dec 1;99(6):1388-1394. [Content Brief]
[4]. Amae S, et al. Identification of a novel isoform of microphthalmia-associated transcription factor that is enriched in retinal pigment epithelium. Biochem Biophys Res Commun. 1998 Jun 29;247(3):710-5. [Content Brief]
[5]. Nardone C, et al. A central role for regulated protein stability in the control of TFE3 and MITF by nutrients. Mol Cell. 2023 Jan 5;83(1):57-73.e9. [Content Brief]
Powered by Bioz
