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Caspase-5 p20 Antibody

(Synonyms: Caspase-5 subunit p20; Apoptosis related cysteine protease; CASP 5; CASP5; Caspase-5; Caspase5; Caspase 5 apoptosis related cysteine peptidase; caspase 5 apoptosis related cysteine protease; ICE; ICE(rel) III; ICE(rel)III; ICEREL III; ICERELIII; ICH 3; ICH 3 protease; ICH3; ICH3 protease; MGC141966; TY; TY protease.)
Customer Review

Based on 1 Customer Validation

Caspase-5 p20 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Caspase-5 p20.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, ELISA

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Caspase-5 p20 Antibody
  • Caspase-5 p20 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-2000 1:100-500 1:100-500 1:100-500 1:5000-10000

Product Details

Description

Caspase-5 p20 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Caspase-5 p20.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse Predicted Reactivity: Rat, Rabbit, Pig, Cow, Dog, Horse
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 50 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 48 kDa
Species Reactivity Database

Entrez Gene: 838 Human ;

SwissProt: P51878 Human ;

Immunogen

KLH conjugated synthetic peptide derived from human Caspase-5 subunit p20: 261-360/434

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103121

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Caspase-5 p20 Antibody
    Western blot analysis of extracts from Hela using Caspase-5 p20 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    Caspase-5 p20 is a Thiol protease that acts as a mediator of programmed cell death. Initiates pyroptosis, a programmed lytic cell death pathway through cleavage of Gasdermin-D (GSDMD): cleavage releases the N-terminal gasdermin moiety (Gasdermin-D, N-terminal) that binds to membranes and forms pores, triggering pyroptosis. Also mediates cleavage and maturation of IL18. Cleavage of GSDMD and IL18 is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4. During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation[1][2][3].

  • Expression


    Tissue_specificity:Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle

    Induction:Up-regulated by bacterial lipopolysaccharides (LPS)

  • Isoforms & Post-Translational Modification

    P51878 has 6 isomers: P51878-1: 49736 Da (predicted); P51878-2: 43261 Da (predicted); P51878-3: 33304 Da (predicted); P51878-4: 12694 Da (predicted); P51878-5: 51177 Da (predicted); P51878-6: 41851 Da (predicted).
    The two subunits are derived from the precursor sequence by an autocatalytic mechanism

  • Subunit

    Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunits (By similarity). Interacts with MEFV (PubMed:17431422). Interacts with SERPINB1; this interaction regulates CASP5 activity (PubMed:30692621)

  • SwissProt ID

    P51878

  • Gene ID
    838 [NCBI]
  • Synonyms

    Caspase-5 subunit p20; Apoptosis related cysteine protease; CASP 5; CASP5; Caspase-5; Caspase5; Caspase 5 apoptosis related cysteine peptidase; caspase 5 apoptosis related cysteine protease; ICE; ICE(rel) III; ICE(rel)III; ICEREL III; ICERELIII; ICH 3; ICH 3 protease; ICH3; ICH3 protease; MGC141966; TY; TY protease.

Caspase-5 p20 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela using Caspase-5 p20 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Caspase-5 p20 Antibody Product Image
    1/16
  • Experimental Validation Results for Caspase-5 p20 Antibody
  • Caspase-5 p20 Antibody Product Image
  • Experimental Validation Results for Caspase-5 p20 Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela using Caspase-5 p20 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Caspase-5 p20 Antibody
  • Caspase-5 p20 Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela using Caspase-5 p20 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Caspase-5 p20 Antibody Product Image WB