Caspase-5 p20 Antibody

Cat. No.: HY-P81252: Data Sheet COA
SDS
User Guide for Antibodies
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Technical Support

Caspase-5 p20 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Caspase-5 p20.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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Caspase-5 p20 Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

Caspase-5 p20 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Caspase-5 p20.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 48 kDa;

Observed band size: 50 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse Predicted Reactivity: Rat, Rabbit, Pig, Cow, Dog, Horse

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

P51878

Gene ID

838 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from human Caspase-5 subunit p20: 261-360/434

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-10000

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela using Caspase-5 p20 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

Function：Thiol protease that acts as a mediator of programmed cell death (PubMed:28314590, PubMed:29898893). Initiates pyroptosis, a programmed lytic cell death pathway through cleavage of Gasdermin-D (GSDMD): cleavage releases the N-terminal gasdermin moiety (Gasdermin-D, N-terminal) that binds to membranes and forms pores, triggering pyroptosis (PubMed:29898893). Also mediates cleavage and maturation of IL18 (PubMed:37993714). Cleavage of GSDMD and IL18 is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4 (PubMed:37993714). During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed:28314590)

Expression：
Tissue_specificity:Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle

Induction:Up-regulated by bacterial lipopolysaccharides (LPS)

Isoforms & Post-Translational Modification：P51878 has 6 isomers: P51878-1: 49736 Da (predicted); P51878-2: 43261 Da (predicted); P51878-3: 33304 Da (predicted); P51878-4: 12694 Da (predicted); P51878-5: 51177 Da (predicted); P51878-6: 41851 Da (predicted).
The two subunits are derived from the precursor sequence by an autocatalytic mechanism

Subunit：Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunits (By similarity). Interacts with MEFV (PubMed:17431422). Interacts with SERPINB1; this interaction regulates CASP5 activity (PubMed:30692621)

RRID

AB_3103121

Database

Entrez Gene: 838 Human ;

SwissProt: P51878 Human ;

OMIM: 602665 Human

Synonyms

Caspase-5 subunit p20; Apoptosis related cysteine protease; CASP 5; CASP5; Caspase-5; Caspase5; Caspase 5 apoptosis related cysteine peptidase; caspase 5 apoptosis related cysteine protease; ICE; ICE(rel) III; ICE(rel)III; ICEREL III; ICERELIII; ICH 3; ICH 3 protease; ICH3; ICH3 protease; MGC141966; TY; TY protease.

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Caspase-5 p20 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Caspase-5 p20 Antibody

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